Open Access

Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer

  • Authors:
    • Juan Du
    • Shu Su
    • Hongyan Li
    • Jie Shao
    • Fanyan Meng
    • Mi Yang
    • Hanqing Qian
    • Zhengyun Zou
    • Xiaoping Qian
    • Baorui Liu
  • View Affiliations

  • Published online on: October 13, 2017     https://doi.org/10.3892/etm.2017.5305
  • Pages:5711-5716
  • Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Adoptive cellular immunotherapy (ACI) has been demonstrated to be a promising cancer therapeutic; however, the inefficient migration of adoptive immune cells to tumors is one of the rate‑limiting factors of ACI. The present study investigated whether 2 Gy low dose irradiation (LDI) was able to increase the migration of adoptive lymphocytes to gastric cancer cells. Treatment with 2 Gy LDI resulted in marked chemokine (C‑X‑C motif) ligand 9 (CXCL9) and CXCL10 production from gastric cancer cell lines. A Transwell chamber migration assay demonstrated enhanced transmigration of cytotoxic T lymphocytes to gastric cancer cells following LDI treatment. After 2 Gy LDI application to established gastric carcinoma in nude mice, labeled immune cells were infused by intravenous injection and concentrated fluorescence signals were observed at the tumor sites within the mice, with a peak signal at 8‑h LDI. Increased numbers of adoptive T cells at the tumor sites were also observed using flow cytometry. Furthermore, a case study of a patient with metastatic gastric cancer who had received ACI treatment combined with 2 Gy LDI provided further evidence that 2 Gy LDI is able to recruit antitumor effector T cells to tumor sites. Therefore, the ability of 2 Gy LDI to convert tumors into inflamed peripheral tissues may be exploited to overcome obstacles at the effector phase of the antitumor immune response and improve the therapeutic efficacy of immunotherapy.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Du, J., Su, S., Li, H., Shao, J., Meng, F., Yang, M. ... Liu, B. (2017). Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer. Experimental and Therapeutic Medicine, 14, 5711-5716. https://doi.org/10.3892/etm.2017.5305
MLA
Du, J., Su, S., Li, H., Shao, J., Meng, F., Yang, M., Qian, H., Zou, Z., Qian, X., Liu, B."Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer". Experimental and Therapeutic Medicine 14.6 (2017): 5711-5716.
Chicago
Du, J., Su, S., Li, H., Shao, J., Meng, F., Yang, M., Qian, H., Zou, Z., Qian, X., Liu, B."Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5711-5716. https://doi.org/10.3892/etm.2017.5305