Open Access

Enhanced osseointegration of titanium implants in a rat model of osteoporosis using multilayer bone mesenchymal stem cell sheets

  • Authors:
    • Yan Duan
    • Wei Ma
    • Dehua Li
    • Tongfei Wang
    • Baolin Liu
  • View Affiliations

  • Published online on: October 13, 2017     https://doi.org/10.3892/etm.2017.5303
  • Pages:5717-5726
  • Copyright: © Duan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate whether bone marrow‑derived mesenchymal stem cell (BMSC) sheets combined with titanium implants enhanced implant osseointegration in an ovariectomized (OVX) rat model of osteoporosis. Sprague‑Dawley rats were randomly assigned into a test group and control group. Allogenic BMSCs were collected from the rats, cultured and stored via cryopreservation. At 6 months post‑ovariectomy, establishment of the OVX model was confirmed by micro‑computed tomography (CT) measurements. BMSC sheets were subsequently layered and wrapped over titanium implants for implantation. Unmodified implants served as the control. At 8 weeks post‑implantation, samples were observed by micro‑CT reconstruction and histomorphometric evaluation. Micro‑CT reconstruction identified a marked improvement in the surrounding bone volume following treatment, with data analyses indicating a significant increase in bone volume in the BMSC‑implant group compared with the control implant group (P<0.05). In addition, histological staining identified new bone formation and an increased rate of bone‑implant contact surrounding the BMSC‑implant constructs. These results indicate that the use of BMSC sheets as a novel tissue engineering approach improves the osseointegration of titanium implants in an osteoporosis model. This method may expand the operative indications in patients with osteoporosis and improve the success rate of clinical dental implant treatments.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

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APA
Duan, Y., Ma, W., Li, D., Wang, T., & Liu, B. (2017). Enhanced osseointegration of titanium implants in a rat model of osteoporosis using multilayer bone mesenchymal stem cell sheets. Experimental and Therapeutic Medicine, 14, 5717-5726. https://doi.org/10.3892/etm.2017.5303
MLA
Duan, Y., Ma, W., Li, D., Wang, T., Liu, B."Enhanced osseointegration of titanium implants in a rat model of osteoporosis using multilayer bone mesenchymal stem cell sheets". Experimental and Therapeutic Medicine 14.6 (2017): 5717-5726.
Chicago
Duan, Y., Ma, W., Li, D., Wang, T., Liu, B."Enhanced osseointegration of titanium implants in a rat model of osteoporosis using multilayer bone mesenchymal stem cell sheets". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5717-5726. https://doi.org/10.3892/etm.2017.5303