Open Access

Trichosanthin enhances the antitumor effect of gemcitabine in non‑small cell lung cancer via inhibition of the PI3K/AKT pathway

  • Authors:
    • Naren Tuya
    • Yadi Wang
    • Lanmei Tong
    • Weishi Gao
    • Rong Yu
    • Liying Xue
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/etm.2017.5286
  • Pages:5767-5772
  • Copyright: © Tuya et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gemcitabine (GEMZ) is the first‑line therapy used against non‑small cell lung cancer (NSCLC), and studies have focused on investigating the potential effects of agents combined with GEMZ to enhance the anticancer efficacy in NSCLC. Previous studies have reported that trichosanthin (TCS) has various physiological and pharmacological effects, including anti‑human influenza virus enzymes, inhibition of protein synthesis and antitumor activity. The purpose of the present study was to investigate if TCS enhanced the antitumor effects of GEMZ in NSCLC. MTT assay demonstrated that TCS significantly enhanced the cytotoxic effect of GEMZ (P>0.05). Furthermore, a propidium iodide/Αnnexin V staining assay revealed that TCS exerted its pharmacological effect by increasing the apoptotic population. In addition, western blot analysis demonstrated that the combination treatment of TCS with GEMZ further decreased the expression level of phosphoinositide 3‑kinase (PI3K) and AKT via regulating the expression of insulin growth factor. The results of the present study demonstrated that TCS enhanced the cytotoxic and apoptotic effects of GEMZ in A549 cells via regulating the PI3K/AKT pathway. In conclusion, these observations may provide a potential rational basis for a combination strategy for chemotherapy treatment of NSCLC.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Tuya, N., Wang, Y., Tong, L., Gao, W., Yu, R., & Xue, L. (2017). Trichosanthin enhances the antitumor effect of gemcitabine in non‑small cell lung cancer via inhibition of the PI3K/AKT pathway. Experimental and Therapeutic Medicine, 14, 5767-5772. https://doi.org/10.3892/etm.2017.5286
MLA
Tuya, N., Wang, Y., Tong, L., Gao, W., Yu, R., Xue, L."Trichosanthin enhances the antitumor effect of gemcitabine in non‑small cell lung cancer via inhibition of the PI3K/AKT pathway". Experimental and Therapeutic Medicine 14.6 (2017): 5767-5772.
Chicago
Tuya, N., Wang, Y., Tong, L., Gao, W., Yu, R., Xue, L."Trichosanthin enhances the antitumor effect of gemcitabine in non‑small cell lung cancer via inhibition of the PI3K/AKT pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5767-5772. https://doi.org/10.3892/etm.2017.5286