Open Access

Anti‑inflammatory effects of Daehwangmokdantang, a traditional herbal formulation, in lipopolysaccharide‑stimulated RAW 264.7 macrophages

  • Authors:
    • Moon Hee Lee
    • Su Hyun Hong
    • Cheol Park
    • Min‑Ho Han
    • Sung Ok Kim
    • Sang Hoon Hong
    • Gi‑Young Kim
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: October 12, 2017     https://doi.org/10.3892/etm.2017.5296
  • Pages:5809-5816
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Daehwangmokdantang (DHMDT) is a traditional polyherbal formulation that has known antidiarrheal and anti‑inflammatory activities. However, the underlying mechanisms of these activities are poorly understood. In the present study, the inhibitory effects of DHMDT on the production of proinflammatory mediators and cytokines in lipopolysaccharide (LPS)‑stimulated RAW 264.7 macrophages were investigated. The inhibitory effects of DHMDT on LPS‑induced nitric oxide (NO), prostaglandin (PG)E2, tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β production were examined using Griess reagent and ELISA detection kits. The effects of DHMDT on the expression of inducible NO synthase (iNOS), cyclooxygenase (COX)‑2, IL‑1β and TNF‑α, and their upstream signal proteins, including nuclear factor (NF)‑κB, mitogen‑activated protein kinases (MAPKs) and RAC‑α serine/threonine‑protein kinase (Akt), a phosphatidylinositol 3‑kinase (PI3K) downstream effector, were investigated using western blotting and immunofluorescence staining. The results revealed the pretreatment with DHMDT significantly inhibited the LPS‑induced production of NO, PGE2, TNF‑α, and IL‑1β, and expression of iNOS, COX‑2 TNF‑α, and IL‑1β, without any significant cytotoxicity. DHMDT also efficiently prevented the translocation of the NF‑κB subunit p65 into the nucleus by interrupting the activation of the upstream mediator inhibitor of NF‑κB kinase α/β. Furthermore, the anti‑inflammatory effects of DHMDT were associated with the suppression of LPS‑induced phosphorylation of Akt and MAPKs in RAW 264.7 macrophages. Therefore, the results of the present study indicate that DHMDT exhibited anti‑inflammatory activity via the inhibition of proinflammatory mediators and cytokines, in which the inactivation of NF‑κB, PI3K/Akt, and MAPKs may be involved. These results suggest that DHMDT may be a potential anti‑inflammatory drug candidate.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Lee, M.H., Hong, S.H., Park, C., Han, M., Kim, S.O., Hong, S.H. ... Choi, Y.H. (2017). Anti‑inflammatory effects of Daehwangmokdantang, a traditional herbal formulation, in lipopolysaccharide‑stimulated RAW 264.7 macrophages. Experimental and Therapeutic Medicine, 14, 5809-5816. https://doi.org/10.3892/etm.2017.5296
MLA
Lee, M. H., Hong, S. H., Park, C., Han, M., Kim, S. O., Hong, S. H., Kim, G., Choi, Y. H."Anti‑inflammatory effects of Daehwangmokdantang, a traditional herbal formulation, in lipopolysaccharide‑stimulated RAW 264.7 macrophages". Experimental and Therapeutic Medicine 14.6 (2017): 5809-5816.
Chicago
Lee, M. H., Hong, S. H., Park, C., Han, M., Kim, S. O., Hong, S. H., Kim, G., Choi, Y. H."Anti‑inflammatory effects of Daehwangmokdantang, a traditional herbal formulation, in lipopolysaccharide‑stimulated RAW 264.7 macrophages". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5809-5816. https://doi.org/10.3892/etm.2017.5296