Open Access

Icaritin inhibits decidualization of endometrial stromal cells

  • Authors:
    • Aiwen Le
    • Zhong Hai Wang
    • Xiao Yun Dai
    • Tian Hui Xiao
    • Rong Zhuo
    • Baozhen Zhang
    • Zhonglin Xiao
    • Xiujun Fan
  • View Affiliations

  • Published online on: October 10, 2017     https://doi.org/10.3892/etm.2017.5278
  • Pages:5949-5955
  • Copyright: © Le et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effects of Icaritin on the proliferation and decidualization of endometrial stromal cells (ESCs). A total of 20 specimens of endometrium were collected during hysterectomy at the Gynecology Department of Shenzhen Nanshan People's Hospital (Shenzhen, China) between August 2014 and December 2015. The endometrium was digested with high concentrations of collagenase and DNase and filtered with meshes, and then the glandular epithelial and stromal cells were separated by the adhesion purification method. The purity of stromal cells was identified by vimetin and cytokeratin 7 immunostaining. The estradiol + progesterone (E2+P4) and/or cyclic adenosine monophosphate (cAMP) were added to induce an in vitro decidualization model, which was used to analyze the effect of Icaritin on the decidualization ability of the human ESCs. The decidualization markers of human ESCs, prolactin (PRL) and insulin‑like growth factor‑binding protein 1 (IGFBP‑1), was analyzed by reverse‑transcription quantitative polymerase chain reaction measurements of the mRNA levels, PRL immunostaining and ELISA analysis of the IGFBP‑1 protein levels in the cells or cell culture supernatant separately. The results demonstrated that treatment with E2+P4 and/or cAMP for 96 h was able to induce decidualization in ESCs, and that the cells demonstrated polygon‑shaped epithelioid changes. The cell nuclei revealed multinuclear changes, and the cells were also observed to be large and round in shape. The PRL expression and upregulated IGFBP‑1 mRNA and protein levels in the E2+P4+cAMP treatment group indicated successful decidualization of the in vitro model. However, the addition of Icaritin inhibited the expression of PRL and IGFBP‑1 mRNA, as well as IGFBP‑1 protein in the induced ESCs compared with groups without Icaritin. These results suggest that Icaritin was able to inhibit the expression of decidualization‑related genes in ESCs in vitro. However, the exact mechanisms require further investigation.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Le, A., Wang, Z.H., Dai, X.Y., Xiao, T.H., Zhuo, R., Zhang, B. ... Fan, X. (2017). Icaritin inhibits decidualization of endometrial stromal cells. Experimental and Therapeutic Medicine, 14, 5949-5955. https://doi.org/10.3892/etm.2017.5278
MLA
Le, A., Wang, Z. H., Dai, X. Y., Xiao, T. H., Zhuo, R., Zhang, B., Xiao, Z., Fan, X."Icaritin inhibits decidualization of endometrial stromal cells". Experimental and Therapeutic Medicine 14.6 (2017): 5949-5955.
Chicago
Le, A., Wang, Z. H., Dai, X. Y., Xiao, T. H., Zhuo, R., Zhang, B., Xiao, Z., Fan, X."Icaritin inhibits decidualization of endometrial stromal cells". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5949-5955. https://doi.org/10.3892/etm.2017.5278