Effectiveness of C5a aptamers in a TNBS‑induced colitis mouse model

  • Authors:
    • Zhiping Li
    • Xiwen Wang
    • Man Chen
    • Yuanyuan Wang
    • Rui Sun
    • Han Qu
    • Yu Sun
    • Weicun Gao
    • Bo Li
    • Xiaolin Dong
    • Yandong Zhang
    • Zhiping Xia
  • View Affiliations

  • Published online on: October 10, 2017     https://doi.org/10.3892/etm.2017.5277
  • Pages:6119-6124
0

Abstract

The complement-activated product, complement component 5a (C5a), is a potent inflammatory peptide with a broad spectrum of functions. In vivo and in vitro studies have demonstrated that C5a serves an important role in inflammation; however, the role of C5a in the pathogenesis of inflammatory bowel disease (IBD) is not known. The purpose of the current study was to investigate the role of C5a in IBD using an experimental mouse model of colitis. Colitis was induced in mice using 2,4,6-trinitrobenzene sulfonic acid (TNBS), and C5a aptamers were subsequently administered via intraperitoneal injection. Clinical symptoms of the disease, histopathological analysis of the colon and the level of inflammatory components were examined. The symptoms of colitis, including changes in behavior, weight loss, colon damage and an increase in inflammatory cytokines, were attenuated following the treatment of mice with TNBS‑induced colitis with C5a aptamers. The aptamer‑treated mice exhibited a marked attenuation of colitis when compared with untreated mice, as demonstrated by the phenotypic observations, histological examinations and inflammatory cytokine levels. Colitis is characterized by an imbalance between pro‑inflammatory and anti‑inflammatory mediators. The results of the current study suggest that C5a may serve a critical role in inflammation in IBD.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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Copy and paste a formatted citation
APA
Li, Z., Wang, X., Chen, M., Wang, Y., Sun, R., Qu, H. ... Xia, Z. (2017). Effectiveness of C5a aptamers in a TNBS‑induced colitis mouse model. Experimental and Therapeutic Medicine, 14, 6119-6124. https://doi.org/10.3892/etm.2017.5277
MLA
Li, Z., Wang, X., Chen, M., Wang, Y., Sun, R., Qu, H., Sun, Y., Gao, W., Li, B., Dong, X., Zhang, Y., Xia, Z."Effectiveness of C5a aptamers in a TNBS‑induced colitis mouse model". Experimental and Therapeutic Medicine 14.6 (2017): 6119-6124.
Chicago
Li, Z., Wang, X., Chen, M., Wang, Y., Sun, R., Qu, H., Sun, Y., Gao, W., Li, B., Dong, X., Zhang, Y., Xia, Z."Effectiveness of C5a aptamers in a TNBS‑induced colitis mouse model". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6119-6124. https://doi.org/10.3892/etm.2017.5277