MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF

  • Authors:
    • Liancheng Zhai
    • Yanli Li
    • Xinzhi Lan
    • Liang Ai
  • View Affiliations

  • Published online on: October 16, 2017     https://doi.org/10.3892/etm.2017.5312
  • Pages:6147-6151
0

Abstract

The aim of the present study was to investigate the effect and mechanism of microRNA (miR)‑10a‑5p in human cervical cancer. The expression level of miR‑10‑5p in cervical cancer lines was assessed using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In cervical cancer HeLa and SiHa cells, miR‑10‑5p was ectopically overexpressed by lentiviral transduction. Brain‑derived neurotrophic factor (BDNF) was then overexpressed in HeLa and SiHa cells to evaluate its selective effect on miR‑10‑5p in cervical cancer modulation. The targeting of miR‑10‑5p on its downstream gene, BDNF, was evaluated using RT‑qPCR and western blot analysis. Cervical cancer cell viability and cell cycle was evaluated using an MTT assay and flow cytometry, respectively. The results indicated that miR‑10‑5p expression was significantly lower in cervical cancer cell lines compared with normal cells (P<0.05). Ectopic overexpression of miR‑10‑5p significantly inhibited cancer cell viability and induced cell cycle arrest in HeLa and SiHa cells (both P<0.05). miR‑10‑5p overexpression significantly reduced BDNF gene expression (P<0.05) and also reduced BDNF protein levels in cervical cancer cells compared with the control. In conclusion, the current study indicated that miR‑10‑5p is a cervical cancer suppressor, which regulates BDNF expression in cervical cancer.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Zhai, L., Li, Y., Lan, X., & Ai, L. (2017). MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF. Experimental and Therapeutic Medicine, 14, 6147-6151. https://doi.org/10.3892/etm.2017.5312
MLA
Zhai, L., Li, Y., Lan, X., Ai, L."MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF". Experimental and Therapeutic Medicine 14.6 (2017): 6147-6151.
Chicago
Zhai, L., Li, Y., Lan, X., Ai, L."MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6147-6151. https://doi.org/10.3892/etm.2017.5312