MicroRNA‑10a‑5p suppresses cancer proliferation and division in human cervical cancer by targeting BDNF
- Liancheng Zhai
- Yanli Li
- Xinzhi Lan
- Liang Ai
Published online on: October 16, 2017
The aim of the present study was to investigate the effect and mechanism of microRNA (miR)‑10a‑5p in human cervical cancer. The expression level of miR‑10‑5p in cervical cancer lines was assessed using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In cervical cancer HeLa and SiHa cells, miR‑10‑5p was ectopically overexpressed by lentiviral transduction. Brain‑derived neurotrophic factor (BDNF) was then overexpressed in HeLa and SiHa cells to evaluate its selective effect on miR‑10‑5p in cervical cancer modulation. The targeting of miR‑10‑5p on its downstream gene, BDNF, was evaluated using RT‑qPCR and western blot analysis. Cervical cancer cell viability and cell cycle was evaluated using an MTT assay and flow cytometry, respectively. The results indicated that miR‑10‑5p expression was significantly lower in cervical cancer cell lines compared with normal cells (P<0.05). Ectopic overexpression of miR‑10‑5p significantly inhibited cancer cell viability and induced cell cycle arrest in HeLa and SiHa cells (both P<0.05). miR‑10‑5p overexpression significantly reduced BDNF gene expression (P<0.05) and also reduced BDNF protein levels in cervical cancer cells compared with the control. In conclusion, the current study indicated that miR‑10‑5p is a cervical cancer suppressor, which regulates BDNF expression in cervical cancer.