HIPK2 inhibits cell metastasis and improves chemosensitivity in esophageal squamous cell carcinoma

  • Authors:
    • Zhen Zhang
    • Penghai Wen
    • Fangfang Li
    • Chuanshan Yao
    • Tongfu Wang
    • Bing Liang
    • Qingle Yang
    • Lei Ma
    • Limin He
  • View Affiliations

  • Published online on: November 8, 2017     https://doi.org/10.3892/etm.2017.5468
  • Pages:1113-1118
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Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive and lethal malignancies worldwide. At present, the underlying mechanisms of ESCC development and progression are poorly understood. Previous studies have demonstrated that homeodomain‑interacting protein kinase‑2 (HIPK2) serves an important role in cancer biology, particularly in proliferation and metastasis. However, the role of HIPK2 in ESCC cells remains unclear. In the current study, the expression of HIPK2 in ESCC specimens, adjacent non‑cancerous tissues and cell lines was assessed using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The effects of HIPK2 on cell metastasis, epithelial‑mesenchymal transition (EMT) and proliferation were studied using a Transwell assay, RT‑qPCR and a Cell Counting Kit‑8 assay, respectively. The results indicated that HIPK2 expression was downregulated in ESCC specimens and cell lines, and HIPK2 expression was associated with tumor invasion and lymph node metastasis. Functional studies demonstrated that HIPK2 overexpression inhibited cell metastasis and EMT. Furthermore, HIPK2 overexpression suppressed cell viability during cisplatin treatment. These results suggest that HIPK2 serves an important role in regulating metastasis and the chemosensitivity of ESCC cells, implicating the potential application of HIPK2 in ESCC therapy.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Zhang, Z., Wen, P., Li, F., Yao, C., Wang, T., Liang, B. ... He, L. (2018). HIPK2 inhibits cell metastasis and improves chemosensitivity in esophageal squamous cell carcinoma. Experimental and Therapeutic Medicine, 15, 1113-1118. https://doi.org/10.3892/etm.2017.5468
MLA
Zhang, Z., Wen, P., Li, F., Yao, C., Wang, T., Liang, B., Yang, Q., Ma, L., He, L."HIPK2 inhibits cell metastasis and improves chemosensitivity in esophageal squamous cell carcinoma". Experimental and Therapeutic Medicine 15.1 (2018): 1113-1118.
Chicago
Zhang, Z., Wen, P., Li, F., Yao, C., Wang, T., Liang, B., Yang, Q., Ma, L., He, L."HIPK2 inhibits cell metastasis and improves chemosensitivity in esophageal squamous cell carcinoma". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1113-1118. https://doi.org/10.3892/etm.2017.5468