Protective effect of baicalin on fetal lung development in a rabbit model of congenital diaphragmatic hernia

Su,Hailong; Mu,Linsong; Liu,Changsheng

doi:10.3892/etm.2017.5409

Protective effect of baicalin on fetal lung development in a rabbit model of congenital diaphragmatic hernia

Authors:
- Hailong Su
- Linsong Mu
- Changsheng Liu
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Affiliations: General Surgery Department, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, General Surgery Department, Weihai Tumor Hospital, Weihai, Shandong 264200, P.R. China
Published online on: October 31, 2017 https://doi.org/10.3892/etm.2017.5409
Pages: 61-66
Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bacalin has been reported to improve fetal lung growth by increasing fetal lung surfactant phospholipids. Therefore, the present study evaluated the effect of transplacental treatment of baicalin during lung development in rabbits with congenital diaphragmatic hernia (DH) rabbit. DH was induced in fetal rabbit on gestational day 23 and baicalin injections were administered daily until term period. Fetal survival rate, body weight and lung‑to‑body weight ratio (LBWR) were subsequently measured, and the expression of surfactant protein B (SPB) and Ki‑67, and morphometry analysis was determined in lung tissues. It was observed that in baicalin treated group the fetal survival rate and LBWR were decreased compared with the control without DH group. Lung morphometry results suggested that treatment with baicalin did not significantly ameliorate congenital DH, and adventitial and medial thickness were similar to those in the control groups, and less muscularization of vessels measuring 30‑60 µm. Furthermore, baicalin treatment did not markedly affect the expressions of Ki‑67 and SPB in fetuses with DH. Baicalin was demonstrated to improve the morphology of lung in rabbits; however, as it did not induce marked airway changes this present study suggests that baicalin is not suitable for the management of congenital DH.

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