Transfection of neurotrophin‑3 into neural stem cells using ultrasound with microbubbles to treat denervated muscle atrophy

Gong,Lin; Jiang,Changqing; Liu,Li; Wan,Shengxiang; Tan,Wen; Ma,Sushuang; Jia,Xiaojian; Wang,Meiwei; Hu,Azhen; Shi,Yu; Zhang,Yu; Shen,Yuanyuan; Wang,Feng; Chen,Yun

doi:10.3892/etm.2017.5439

Transfection of neurotrophin‑3 into neural stem cells using ultrasound with microbubbles to treat denervated muscle atrophy

Authors:
- Lin Gong
- Changqing Jiang
- Li Liu
- Shengxiang Wan
- Wen Tan
- Sushuang Ma
- Xiaojian Jia
- Meiwei Wang
- Azhen Hu
- Yu Shi
- Yu Zhang
- Yuanyuan Shen
- Feng Wang
- Yun Chen
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Affiliations: Department of Ultrasound, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Department of Sports Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Shenzhen Key Laboratory for Drug Addiction and Medication Safety, Biomedical Research Institute, Shenzhen Peking University, The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China, Department of Ultrasound, The Third People's Hospital of Shenzhen, Shenzhen, Guangdong 518055, P.R. China, Department of Biomedical Engineering, National Regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China
Published online on: November 6, 2017 https://doi.org/10.3892/etm.2017.5439
Pages: 620-626
Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Neurotrophin-3 (NT-3) has potential as a therapeutic agent for the treatment of patients with denervated muscle atrophy. However, the endogenous secretion of NT‑3 is low and exogenous NT‑3 lacks sufficient time to accumulate due to its short half‑life. The transfection of NT‑3 has been demonstrated to have a beneficial effect on denervated muscle and motor endplates. Neural stem cells (NSCs) differentiate into neurons and form motor endplate nerve‑muscle connections. It has been previously demonstrated that local and noninvasive transfection can be performed using ultrasound with microbubbles (MBs). In the current study, hematoxylin and eosin, acetylcholinesterase and gold chloride staining, as well as transmission electron microscopy, were performed to verify the effects of this treatment strategy. The results demonstrated that using ultrasound with MBs for the transfection of NT‑3 into NSCs, and their subsequent transplantation in vivo, attenuated the atrophy of denervated muscle and reduced motor endplate degeneration. This noninvasive, efficient and targeted treatment strategy may therefore be a potential treatment for patients with denervated muscle atrophy.

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