Open Access

COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle‑related proteins

  • Authors:
    • Yan Wang
    • Zhi Chai
    • Min Wang
    • Yanling Jin
    • Aijun Yang
    • Min Li
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/etm.2017.5506
  • Pages:777-784
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Coat proteins (COPs), including the major types clathrin, COPI and COPII, play a considerable role in intracellular transport by initiating the formation of transport vesicles. Coatomer protein complex subunit β2 (COPB2) is one of the seven subunits that make up a COPI complex. In the present study, we found that COPB2 was highly expressed in human colon cancer specimens. However, to date, there have been no reports describing the functions of COPB2 in human colon cancer cells. In this study, we analyzed the functions of COPB2 in the proliferation and cell cycle arrest of human RKO and HCT116 colon cancer cells by using lentivirus‑mediated RNAi infection. Our results demonstrated that the silencing of COPB2 in vitro could inhibit the proliferation and colony formation abilities of RKO and HCT116 cells. Furthermore, measurement of cell cycle distribution indicated that the downregulation of COPB2 could induce G0/G1 or S phase cell cycle arrest by regulating cell cycle‑related proteins. In conclusion, our results suggest that COPB2 plays a key role in the proliferation and cell cycle progression of human RKO and HCT116 colon cancer cells, thus indicating that COPB2 might be a potential therapeutic target for the treatment of human colon cancer.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Wang, Y., Chai, Z., Wang, M., Jin, Y., Yang, A., & Li, M. (2018). COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle‑related proteins. Experimental and Therapeutic Medicine, 15, 777-784. https://doi.org/10.3892/etm.2017.5506
MLA
Wang, Y., Chai, Z., Wang, M., Jin, Y., Yang, A., Li, M."COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle‑related proteins". Experimental and Therapeutic Medicine 15.1 (2018): 777-784.
Chicago
Wang, Y., Chai, Z., Wang, M., Jin, Y., Yang, A., Li, M."COPB2 suppresses cell proliferation and induces cell cycle arrest in human colon cancer by regulating cell cycle‑related proteins". Experimental and Therapeutic Medicine 15, no. 1 (2018): 777-784. https://doi.org/10.3892/etm.2017.5506