Open Access

Early growth response factor‑1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats

  • Authors:
    • Shi Wang
    • Haipeng Dong
    • Chengwei Liu
    • Guichao Xu
    • Xinhua Hu
    • Yichuan Fan
    • Liting Chen
  • View Affiliations

  • Published online on: May 8, 2018     https://doi.org/10.3892/etm.2018.6139
  • Pages: 141-148
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to characterize the effects of early growth response factor‑1 DNA enzyme (EDRz) in a rat abdominal aortic aneurysm (AAA) model to determine the mechanism by which EDRz inhibits AAA and affects the formation of AAA by regulating the activity of matrix metalloproteinase (MMP)‑2 and MMP‑9. EDRz was transfected into the abdominal aorta of rats using the jetPRIME transfection reagent following infusion with elastase. Fluorescent microscopy, hematoxylin and eosin staining, ultrastructural analysis, reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemical analysis were performed to characterize the response to EDRz. The EDRz group showed minimal aneurysm formation when compared with the control group, with significantly lower aortic diameter expansion (2.5±0.1 vs. 3.5±0.1 mm; P<0.05). Early growth response factor 1 (Egr‑1) mRNA and protein levels were significantly decreased in the EDRz group, as expected. The decrease in Egr‑1 was accompanied by decreases in the mRNA and protein levels of MMP‑2 and MMP‑9 (P<0.05). Transfection of the Egr‑1 specific synthetic DNA enzyme EDRz significantly reduced AAA following elastase infusion in rats, at least in part due to the decreased expression of downstream MMP‑2 and MMP‑9.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang S, Dong H, Liu C, Xu G, Hu X, Fan Y and Chen L: Early growth response factor‑1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats. Exp Ther Med 16: 141-148, 2018.
APA
Wang, S., Dong, H., Liu, C., Xu, G., Hu, X., Fan, Y., & Chen, L. (2018). Early growth response factor‑1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats. Experimental and Therapeutic Medicine, 16, 141-148. https://doi.org/10.3892/etm.2018.6139
MLA
Wang, S., Dong, H., Liu, C., Xu, G., Hu, X., Fan, Y., Chen, L."Early growth response factor‑1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats". Experimental and Therapeutic Medicine 16.1 (2018): 141-148.
Chicago
Wang, S., Dong, H., Liu, C., Xu, G., Hu, X., Fan, Y., Chen, L."Early growth response factor‑1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats". Experimental and Therapeutic Medicine 16, no. 1 (2018): 141-148. https://doi.org/10.3892/etm.2018.6139