Open Access

Hydrogen attenuated oxidized low‑density lipoprotein‑induced inflammation through the stimulation of autophagy via sirtuin 1

  • Authors:
    • Sen Yang
    • Ju He
    • Xiaofeng Li
    • Hui Liu
    • Jian Zhao
    • Mingming Liu
  • View Affiliations

  • Published online on: September 4, 2018     https://doi.org/10.3892/etm.2018.6691
  • Pages: 4042-4048
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic inflammation is a central pathogenic mechanism underlying the induction and progression of atherosclerosis (AS). Hydrogen has been demonstrated to serve a protective role in diverse models of disease. However, the potential effects and mechanism of hydrogen with respect to ox‑LDL‑induced inflammation have not yet been completely elucidated. In the present study, various concentrations (0, 50 and 100 mg/l) of oxidized low‑density lipoprotein (ox‑LDL) were used to treat RAW264.7 cells. A Cell Counting kit‑8 assay was used to determine cell viability and western blot analysis was performed to determine the expression of proteins that are involved in autophagy. The expression of inflammatory cytokines in ox‑LDL‑treated macrophages was detected using ELISA. Small interfering (si)RNA against sirtuin 1 (SIRT1) was employed to investigate the mechanism underlying hydrogen‑activated autophagy. The results indicated that ox‑LDL stimulation promoted inflammatory cytokine expression and impaired autophagic flux in RAW264.7 cells. Furthermore, hydrogen inhibited ox‑LDL‑induced inflammatory cytokine expression by upregulating autophagic flux. SIRT1 mediated the upregulation of autophagic flux via hydrogen in ox‑LDL‑treated macrophages. To conclude, the present study provided novel insights into the role of defective autophagy in the pathogenesis of AS and identified autophagy to be a promising therapeutic target for the treatment of AS. Notably, hydrogen may represent a potential agent for the treatment of AS.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yang S, He J, Li X, Liu H, Zhao J and Liu M: Hydrogen attenuated oxidized low‑density lipoprotein‑induced inflammation through the stimulation of autophagy via sirtuin 1. Exp Ther Med 16: 4042-4048, 2018
APA
Yang, S., He, J., Li, X., Liu, H., Zhao, J., & Liu, M. (2018). Hydrogen attenuated oxidized low‑density lipoprotein‑induced inflammation through the stimulation of autophagy via sirtuin 1. Experimental and Therapeutic Medicine, 16, 4042-4048. https://doi.org/10.3892/etm.2018.6691
MLA
Yang, S., He, J., Li, X., Liu, H., Zhao, J., Liu, M."Hydrogen attenuated oxidized low‑density lipoprotein‑induced inflammation through the stimulation of autophagy via sirtuin 1". Experimental and Therapeutic Medicine 16.5 (2018): 4042-4048.
Chicago
Yang, S., He, J., Li, X., Liu, H., Zhao, J., Liu, M."Hydrogen attenuated oxidized low‑density lipoprotein‑induced inflammation through the stimulation of autophagy via sirtuin 1". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4042-4048. https://doi.org/10.3892/etm.2018.6691