microRNA-99b acts as a tumor suppressor in non-small cell lung cancer by directly targeting fibroblast growth factor receptor 3

  • Authors:
    • JaeKu Kang
    • Soo Young Lee
    • Sun Young Lee
    • Young Jin Kim
    • Jae Yong Park
    • Sun Jung Kwon
    • Moon Jun Na
    • Eun Jin Lee
    • Hyo Sung Jeon
    • Ji Woong Son
  • View Affiliations

  • Published online on: October 14, 2011     https://doi.org/10.3892/etm.2011.366
  • Pages: 149-153
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Abstract

microRNAs (miRNAs) play a significant role in cancer development and progression by regulating the expression of proto-oncogenes or tumor suppressor genes. Our previous study using microarrays demonstrated that miR-99b was downregulated in patients with lung cancer. To assess whether or not miR-99b has a functional role in lung cancer, we determined the expression of miR-99b and fibroblast growth factor receptor 3 (FGFR3), which is a predicted target of miR-99b in public algorithms in human lung cancer tissues. miR-99b was downregulated and FGFR3 was upregulated in lung cancer patients. We demonstrated that the overexpression of miR-99b induced a reduction in FGFR3 expression and confirmed the target specificity between miR-99b and the FGFR3 3'-untranslated region by luciferase reporter assay. In addition, the growth rate in miR-99b precursor-treated cells was lower compared to the negative controls. Taken together, these results suggest that miR-99b may be a tumor suppressor through the downregulation of FGFR3. miR-99b may be a potent tumor suppressor and may be a potential therapeutic tool for patients with lung cancer.
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January 2012
Volume 3 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kang J, Lee S, Lee S, Kim Y, Park J, Kwon S, Na M, Lee E, Jeon H, Son J, Son J, et al: microRNA-99b acts as a tumor suppressor in non-small cell lung cancer by directly targeting fibroblast growth factor receptor 3. Exp Ther Med 3: 149-153, 2012
APA
Kang, J., Lee, S., Lee, S., Kim, Y., Park, J., Kwon, S. ... Son, J. (2012). microRNA-99b acts as a tumor suppressor in non-small cell lung cancer by directly targeting fibroblast growth factor receptor 3. Experimental and Therapeutic Medicine, 3, 149-153. https://doi.org/10.3892/etm.2011.366
MLA
Kang, J., Lee, S., Lee, S., Kim, Y., Park, J., Kwon, S., Na, M., Lee, E., Jeon, H., Son, J."microRNA-99b acts as a tumor suppressor in non-small cell lung cancer by directly targeting fibroblast growth factor receptor 3". Experimental and Therapeutic Medicine 3.1 (2012): 149-153.
Chicago
Kang, J., Lee, S., Lee, S., Kim, Y., Park, J., Kwon, S., Na, M., Lee, E., Jeon, H., Son, J."microRNA-99b acts as a tumor suppressor in non-small cell lung cancer by directly targeting fibroblast growth factor receptor 3". Experimental and Therapeutic Medicine 3, no. 1 (2012): 149-153. https://doi.org/10.3892/etm.2011.366