Magnetic resonance imaging‑navigated argon‑helium cryoablation therapy against a rabbit VX2 brain tumor

Wang,Yang; Kan,Hong‑Li; Sun,Hong; Wang,Dong‑Xin; Wang,Huai‑Wu; Liu,Ji‑Xin

doi:10.3892/etm.2015.2375

Magnetic resonance imaging‑navigated argon‑helium cryoablation therapy against a rabbit VX2 brain tumor

Authors:
- Yang Wang
- Hong‑Li Kan
- Hong Sun
- Dong‑Xin Wang
- Huai‑Wu Wang
- Ji‑Xin Liu
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Affiliations: Department of Interventional Oncology, Jilin Provincial People's Hospital, Changchun, Jilin 130021, P.R. China, Department of Anesthesia, Tumor Hospital of Jilin Province, Changchun, Jilin 130012, P.R. China
Published online on: March 20, 2015 https://doi.org/10.3892/etm.2015.2375
Pages: 2229-2234

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Abstract

The aim of the present study was to investigate the feasibility of interventional magnetic resonance imaging (MRI)‑guided and monitored argon‑helium cryoablation for the treatment of brain tumors in rabbits. In addition, the present study evaluated the associations between imaging and pathology, the therapeutic effects and the effects on the surrounding normal tissues. A total of 14 rabbits were equally divided into groups C and D. Under general anesthesia, the skull was drilled and tumor blocks were implanted. Subsequently, a New Zealand rabbit VX2 brain tumor model was successfully established. Rabbits in group C were treated with argon‑helium cryoablation and those in group D did not undergo any treatment (control). Regular postoperative MRI scanning was performed to observe the changes in tumor size, and the survival times of the rabbits in groups C and D were recorded. The extent of necrosis in the brain tumor exhibited a significant correlation with the freezing time of cryoablation, and the necrotic region was shown to be the same size as the ice ball. The survival times of the rabbits in the treatment group (group C) were significantly prolonged. Therefore, the observations of the present study demonstrated that the VX2 brain tumor model, produced using an improved tumor block implantation method, was stable and suitable for MRI observation and interventional study. In addition, argon‑helium cryoablation was shown to be a safe and feasible therapeutic method for the treatment of brain tumors, and was demonstrated to significantly increase the survival times of the brain tumor‑bearing rabbits.

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1	Katchy KC, Alexander S, Al-Nashmi NM and Al-Ramadan A: Epidemiology of primary brain tumors in childhood and adolescence in Kuwait. Springerplus. 2:582013. View Article : Google Scholar : PubMed/NCBI
2	Ziegler DS, Cohn RJ, McCowage G, et al: Efficacy of vincristine and etoposide with escalating cyclophosphamide in poor-prognosis pediatric brain tumors. Neuro Oncol. 8:53–59. 2006. View Article : Google Scholar : PubMed/NCBI
3	Lehotska V, Tothova L and Valkovic L: The role of dynamic contrast-enhanced MRI in differentiation of local recurrence and residual soft-tissue tumor versus post-treatment changes. Bratisl Lek Listy. 114:88–92. 2013.PubMed/NCBI
4	Liu JG, Chen FL, Ge CL, Gong MY, Zuo HB and Zhang JR: Cryosurgery for treatment of subcutaneously xenotransplanted tumors in rats and its effect on cellular immunity. Technol Cancer Res Treat. 10:339–346. 2011.PubMed/NCBI
5	Yang Y, Wang C, Lu Y, et al: Outcomes of ultrasound-guided percutaneous argon-helium cryoablation of hepatocellular carcinoma. J Hepatobiliary Pancreat Sci. 19:674–684. 2012. View Article : Google Scholar : PubMed/NCBI
6	Vasil'ev SA, Pesnia-Prasolov SB, Krylov VV, et al: Cryodestruction in neurosurgery. Khirurgiia (Mosk). 2:105–108. 2013.[(In Russian)]. PubMed/NCBI
7	Bomers JG, Yakar D, Overduin CG, et al: MR imaging-guided focal cryoablation in patients with recurrent prostate cancer. Radiology. 268:451–460. 2013. View Article : Google Scholar : PubMed/NCBI
8	Jaskólski DJ, Fortuniak J, Majos A, et al: Magnetic resonance spectroscopy in intracranial tumours of glial origin. Neurol Neurochir Pol. 47:438–449. 2013.PubMed/NCBI
9	Sun CJ, Li C, Yu JM, et al: Comparison of 64-slice CT perfusion imaging with contrast-enhanced CT for evaluating the target volume for three-dimensional conformal radiotherapy in the rabbit VX 2 brain tumor model. J Radiat Res. 53:454–461. 2012.PubMed/NCBI
10	Bahn D, de Castro Abreu AL, Gill IS, et al: Focal cryotherapy for clinically unilateral low-intermediate risk prostate cancer in 73 men with a median follow-up of 3.7 years. Eur Urol. 62:55–63. 2012. View Article : Google Scholar : PubMed/NCBI
11	Gangi A, Tsoumakidou G, Abdelli O, et al: Percutaneous MR-guided cryoablation of prostate cancer: initial experience. Eur Radiol. 22:1829–1835. 2012. View Article : Google Scholar : PubMed/NCBI
12	Anayama T, Nakajima T, Dunne M, et al: A novel minimally invasive technique to create a rabbit vX2 lung tumor model for nano-sized image contrast and interventional studies. PLoS One. 8:e673552013. View Article : Google Scholar : PubMed/NCBI
13	He Y, Tu HJ, Lei Z, et al: Experimental study of apoptosis in C6 rat glioma model induced by cryotherapy. Zhonghua Zhong Liu Fang Zhi Za Zhi. 12:1615–1618. 2005.[(In Chinese)].
14	Song J, Li C, Wu L, et al: MRI-guided brain tumor cryoablation in a rabbit model. J Magn Reson Imaging. 29:545–551. 2009. View Article : Google Scholar : PubMed/NCBI
15	Mei LJ, Yang XJ, Tang L, Hassan AH, Yonemura Y and Li Y: Establishment and identification of a rabbit model of peritoneal carcinomatosis from gastric cancer. BMC Cancer. 10:1242010. View Article : Google Scholar : PubMed/NCBI
16	Handal JA, Schulz JF, Florez GB, Kwok SC, Khurana JS and Samuel SP: Creation of rabbit bone and soft tissue tumor using cultured VX2 cells. J Surg Res. 179:e127–e132. 2013. View Article : Google Scholar : PubMed/NCBI
17	Anderson JK, Shingleton WB and Cadeddu JA: Imaging associated with percutaneous and intraoperative management of renal tumors. Urol Clin North Am. 33:339–352. 2006. View Article : Google Scholar : PubMed/NCBI
18	Tacke J, Speetzen R, Adam G, et al: Experimental MR imaging-guided interstitial cryotherapy of the brain. AJNR Am J Neuroradiol. 22:431–440. 2001.PubMed/NCBI
19	Hu KW, Li QW, Zuo MH, Sun T and Jiang M: Clinical observation on the combined treatment of 57 cases of non-small cell lung cancer using argon-helium cryosurgery and C hinese herbal medicine. Chin J Integr Med. 13:224–227. 2007. View Article : Google Scholar : PubMed/NCBI
20	Mack MG and Vogl TJ: MR-guided ablation of head and neck tumors. Neuroimaging Clin N Am. 14:853–859. 2004. View Article : Google Scholar : PubMed/NCBI
21	Xiao H, Liao Q, Cheng M, et al: 5-Amino-4-oxopentanoic acid photodynamic diagnosis guided microsurgery and photodynamic therapy on VX 2 brain tumour implanted in a rabbit model. Chin Med J (Engl). 122:1316–1321. 2009.PubMed/NCBI