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Detection of cancer cells in the blood by FACS sorting of CD45- cells.

  • Authors:
    • J Baran
    • A Pituch-Noworolska
    • A Krzeszowiak
    • J Wieckiewicz
    • J Stachura
    • J Pryjma
    • T Popiela
    • A Szczepanik
    • M Zembala

  • View Affiliations

  • Published online on: March 1, 1998     https://doi.org/10.3892/ijmm.1.3.573
  • Pages: 573-581
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Abstract

We describe a simple and sensitive method for detection of low number of cancer cells in the blood. The method is based on FACS sorting of leukocytes labelled with anti-CD45 monoclonal antibody and examining CD45- cells by conventional cytology and immunostaining for cytokeratin 18. In a model study, cancer cells seeded at the frequency of 1 per 106 and 1 per 107 leukocytes were detected in CD45- population. Sensitivity of this method was comparable to reverse transcription polymerase chain reaction (RT-PCR) used for detection of cancer cells expressing CD44 variants-mRNA. In a pilot study, cancer cells were also isolated from the blood of some patients with locally advanced gastric cancer. This method may be useful for detection of circulating tumour cells in cancer patients.

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Journal Cover

Mar 1998
Volume 1 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Baran J, Pituch-Noworolska A, Krzeszowiak A, Wieckiewicz J, Stachura J, Pryjma J, Popiela T, Szczepanik A and Zembala M: Detection of cancer cells in the blood by FACS sorting of CD45- cells.. Int J Mol Med 1: 573-581, 1998
APA
Baran, J., Pituch-Noworolska, A., Krzeszowiak, A., Wieckiewicz, J., Stachura, J., Pryjma, J. ... Zembala, M. (1998). Detection of cancer cells in the blood by FACS sorting of CD45- cells.. International Journal of Molecular Medicine, 1, 573-581. https://doi.org/10.3892/ijmm.1.3.573
MLA
Baran, J., Pituch-Noworolska, A., Krzeszowiak, A., Wieckiewicz, J., Stachura, J., Pryjma, J., Popiela, T., Szczepanik, A., Zembala, M."Detection of cancer cells in the blood by FACS sorting of CD45- cells.". International Journal of Molecular Medicine 1.3 (1998): 573-581.
Chicago
Baran, J., Pituch-Noworolska, A., Krzeszowiak, A., Wieckiewicz, J., Stachura, J., Pryjma, J., Popiela, T., Szczepanik, A., Zembala, M."Detection of cancer cells in the blood by FACS sorting of CD45- cells.". International Journal of Molecular Medicine 1, no. 3 (1998): 573-581. https://doi.org/10.3892/ijmm.1.3.573