Epidermal growth factor downregulates the expression of SH-PTP2.

  • Authors:
    • A R Kamer
    • S A Hoghooghi
    • C Liebow
  • View Affiliations

  • Published online on: April 1, 1998     https://doi.org/10.3892/ijmm.1.4.735
  • Pages: 735-744
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Abstract

Protein phosphorylation/dephosphorylation on tyrosine residues are regulated by tyrosine kinases/phosphatases. The tyrosine phosphatase SH-PTP2 (PTP1D, PTP2C) interacts physically through its SH2 domain with phosphorylated epidermal growth factor receptor (EGFR). In KB cells, an oral epidermoid carcinoma, high epidermal growth factor (EGF) concentrations (10-9, 10-8 and 10-7 M) downregulate its receptor for the duration of the incubation with EGF. Thus, it was hypothesized that in KB cells, SH-PTP2 expression would also be downregulated by high EGF concentrations. This hypothesis was investigated by incubating the KB cells with increasing concentrations of EGF (0, 10-11, 10-10, 10-9, 10-8, 10-7 M) and by evaluating the expression of SH-PTP2 protein under these conditions. This study showed that EGF 10-7 and 10-8 M significantly decreased SH-PTP2 protein expression compared to controls. EGF 10-10 and 10-11 M did not change the expression of SH-PTP2 protein. We conclude that high EGF concentrations downregulate the expression of SH-PTP2 protein.

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Apr 1998
Volume 1 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kamer A, Hoghooghi S and Liebow C: Epidermal growth factor downregulates the expression of SH-PTP2.. Int J Mol Med 1: 735-744, 1998.
APA
Kamer, A., Hoghooghi, S., & Liebow, C. (1998). Epidermal growth factor downregulates the expression of SH-PTP2.. International Journal of Molecular Medicine, 1, 735-744. https://doi.org/10.3892/ijmm.1.4.735
MLA
Kamer, A., Hoghooghi, S., Liebow, C."Epidermal growth factor downregulates the expression of SH-PTP2.". International Journal of Molecular Medicine 1.4 (1998): 735-744.
Chicago
Kamer, A., Hoghooghi, S., Liebow, C."Epidermal growth factor downregulates the expression of SH-PTP2.". International Journal of Molecular Medicine 1, no. 4 (1998): 735-744. https://doi.org/10.3892/ijmm.1.4.735