Telomerase activity in malignant and benign bladder conditions.

  • Authors:
    • M P Mayfield
    • T Shah
    • G M Flannigan
    • P A Hamilton Stewart
    • M C Bibby
  • View Affiliations

  • Published online on: May 1, 1998     https://doi.org/10.3892/ijmm.1.5.835
  • Pages: 835-875
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Abstract

This study investigated whether the enzyme telomerase is active in bladder tumours, whether there is any correlation between activity and grade, and whether the enzyme is expressed in non-malignant conditions. Fifty-two patients undergoing cystoscopy or TURBT at a district general hospital were included, 25 with current bladder tumours, 13 with previous but no current tumours, and 14 with non-malignant pathology. Specimens were analysed by the telomerase repeat amplification protocol (TRAP assay), a highly-sensitive polymerase chain reaction (PCR)-based assay, and a commercially-available ELISA kit. Telomerase activity was detected in 80% of bladder tumours, more frequently in moderate- or poorly-differentiated (93%) than well-differentiated (56%) tumours. Activity was not uniform across individual tumours. Telomerase was also frequently (61%) detected in inflammatory lesions found in patients being followed up for previous bladder tumours, and in two (14%) patients with benign pathologies. In conclusion, telomerase was frequently but not uniformly detected in bladder tumours; its presence was not specific to malignancy. There is a possible correlation between tumour grade and telomerase activity.

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May 1998
Volume 1 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Mayfield M, Shah T, Flannigan G, Hamilton Stewart P and Bibby M: Telomerase activity in malignant and benign bladder conditions.. Int J Mol Med 1: 835-875, 1998.
APA
Mayfield, M., Shah, T., Flannigan, G., Hamilton Stewart, P., & Bibby, M. (1998). Telomerase activity in malignant and benign bladder conditions.. International Journal of Molecular Medicine, 1, 835-875. https://doi.org/10.3892/ijmm.1.5.835
MLA
Mayfield, M., Shah, T., Flannigan, G., Hamilton Stewart, P., Bibby, M."Telomerase activity in malignant and benign bladder conditions.". International Journal of Molecular Medicine 1.5 (1998): 835-875.
Chicago
Mayfield, M., Shah, T., Flannigan, G., Hamilton Stewart, P., Bibby, M."Telomerase activity in malignant and benign bladder conditions.". International Journal of Molecular Medicine 1, no. 5 (1998): 835-875. https://doi.org/10.3892/ijmm.1.5.835