Interactions between human monocytes and fibronectin are suppressed by interferons β and γ, but not α: Correlation with Rho-paxillin signaling

  • Authors:
    • Brigitte Surin
    • Dany Rouillard
    • Brigitte Bauvois
  • View Affiliations

  • Published online on: July 1, 2002     https://doi.org/10.3892/ijmm.10.1.25
  • Pages: 25-31
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Modulation of the adhesive responses of monocytic cells may reflect their motility at sites of diseased tissues (inflammation, tumors). Integrins α5β1 mediate fibronectin (Fn)-dependent adhesion of human monocytes and their precursors. The effect of type I IFNs (α, β) and type II IFN (γ) was assessed on the adhesive capacities of promonocytic U937 cells and monocytes. IFN-β and IFN-γ abrogated monocytic cell adhesion to Fn, but such impaired cell attachment was not due to altered levels of α5β1 integrins. In contrast, IFN-α did not affect cell adhesion to Fn. Participation of cytoskeleton assembly in IFN-mediated cell detachment was evaluated. Activation of RhoA activity with lysophosphatidic acid (LPA) increased 2-fold the adhesion of monocytic cells to Fn in a α5β1-mediated fashion, and IFN-γ treatment reversed the enhancing effect of LPA. Moreover, U937 cells and monocytes dominantly expressed the 44-46 kDa paxillin forms and IFN-β and IFN-γ led to the accumulation of 66-70 kDa paxillin forms. These results indicate that IFN-mediated loss of monocyte adhesion to Fn is associated with changes in the cytoskeleton associated proteins paxillin and Rho.

Related Articles

Journal Cover

July 2002
Volume 10 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Surin B, Rouillard D and Bauvois B: Interactions between human monocytes and fibronectin are suppressed by interferons β and γ, but not α: Correlation with Rho-paxillin signaling. Int J Mol Med 10: 25-31, 2002.
APA
Surin, B., Rouillard, D., & Bauvois, B. (2002). Interactions between human monocytes and fibronectin are suppressed by interferons β and γ, but not α: Correlation with Rho-paxillin signaling. International Journal of Molecular Medicine, 10, 25-31. https://doi.org/10.3892/ijmm.10.1.25
MLA
Surin, B., Rouillard, D., Bauvois, B."Interactions between human monocytes and fibronectin are suppressed by interferons β and γ, but not α: Correlation with Rho-paxillin signaling". International Journal of Molecular Medicine 10.1 (2002): 25-31.
Chicago
Surin, B., Rouillard, D., Bauvois, B."Interactions between human monocytes and fibronectin are suppressed by interferons β and γ, but not α: Correlation with Rho-paxillin signaling". International Journal of Molecular Medicine 10, no. 1 (2002): 25-31. https://doi.org/10.3892/ijmm.10.1.25