Molecular cloning, mapping and characterization of a human CK1γ1 gene

  • Authors:
    • Lingchen Guo
    • Min Jiang
    • Yushu Ma
    • Haipeng Cheng
    • Xiaohua Ni
    • Yuhong Ma
    • Chaoneng Ji
    • Yi Xie
    • Yumin Mao
  • View Affiliations

  • Published online on: August 1, 2002     https://doi.org/10.3892/ijmm.10.2.227
  • Pages: 227-230
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Abstract

We isolated and sequenced a cDNA clone coding a human protein kinase CK1 (casein kinase 1) by screening a human fetal brain cDNA library. This new cDNA clone of 1756 bp contained an open reading frame, encoding a protein of 438 amino acids with a molecular weight of 50,272 Da and an isoelectronic point of 9.37. The entire amino acid sequence of the novel human CK1 was 94% homologous to that of rat CK1γ1. Northern blot analysis indicated that the human CK1γ1 was highly expressed in the liver, skeletal muscle, heart and kidney. Furthermore, the human CK1γ1 gene was mapped to chromosome 15q22 between STS marker D15S159 and D15S125 by polymerase chain reaction analysis of human/rodent hybrid cell panels.

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August 2002
Volume 10 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Guo L, Jiang M, Ma Y, Cheng H, Ni X, Ma Y, Ji C, Xie Y and Mao Y: Molecular cloning, mapping and characterization of a human CK1γ1 gene. Int J Mol Med 10: 227-230, 2002
APA
Guo, L., Jiang, M., Ma, Y., Cheng, H., Ni, X., Ma, Y. ... Mao, Y. (2002). Molecular cloning, mapping and characterization of a human CK1γ1 gene. International Journal of Molecular Medicine, 10, 227-230. https://doi.org/10.3892/ijmm.10.2.227
MLA
Guo, L., Jiang, M., Ma, Y., Cheng, H., Ni, X., Ma, Y., Ji, C., Xie, Y., Mao, Y."Molecular cloning, mapping and characterization of a human CK1γ1 gene". International Journal of Molecular Medicine 10.2 (2002): 227-230.
Chicago
Guo, L., Jiang, M., Ma, Y., Cheng, H., Ni, X., Ma, Y., Ji, C., Xie, Y., Mao, Y."Molecular cloning, mapping and characterization of a human CK1γ1 gene". International Journal of Molecular Medicine 10, no. 2 (2002): 227-230. https://doi.org/10.3892/ijmm.10.2.227