GnRH agonist inhibits human telomerase reverse transcriptase mRNA expression in endometrial cancer cells

  • Authors:
    • N. Nagai
    • T. Oshita
    • K. Mukai
    • Y. Shiroyama
    • K. Shigemasa
    • K. Ohama
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijmm.10.5.593
  • Pages: 593-597
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Abstract

We investigated the relationship between the antiproliferative effect of GnRH agonist and telomerase activity using the endometrial cancer cell line HEC-1A. The subjects were 38 endometrial cancer, and 2 atypical endometrial hyperplasia patients. GnRH-R expression was detected using RT-PCR. HEC-1A cells were incubated with 10-7-10-4 M GnRH agonist (leuprolide acetate), and cell proliferation was determined using MTT assay. The telomerase activity was detected by the TRAP assay and expression of human telomerase reverse transcriptase (hTERT) was assessed by RT-PCR. GnRH-R mRNA was detected at 94.7% (36/38) in endometrial cancer and in both of the atypical endometrial hyperplasia and in HEC-1A cells. Cell proliferation of HEC-1A showed significant inhibition at leuprolide acetate concentrations of 10-6 M or higher compared with untreated control culture (p<0.05). The telomerase activity showed no marked difference compared with untreated culture. However, hTERT mRNA expression showed a decrease in the leuprolide-treated cells. It is suggested that the mechanism of the antitumor effect of GnRH agonist involved the inhibition of hTERT mRNA expression in the endometrial cancer cells.

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November 2002
Volume 10 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nagai N, Oshita T, Mukai K, Shiroyama Y, Shigemasa K and Ohama K: GnRH agonist inhibits human telomerase reverse transcriptase mRNA expression in endometrial cancer cells. Int J Mol Med 10: 593-597, 2002
APA
Nagai, N., Oshita, T., Mukai, K., Shiroyama, Y., Shigemasa, K., & Ohama, K. (2002). GnRH agonist inhibits human telomerase reverse transcriptase mRNA expression in endometrial cancer cells. International Journal of Molecular Medicine, 10, 593-597. https://doi.org/10.3892/ijmm.10.5.593
MLA
Nagai, N., Oshita, T., Mukai, K., Shiroyama, Y., Shigemasa, K., Ohama, K."GnRH agonist inhibits human telomerase reverse transcriptase mRNA expression in endometrial cancer cells". International Journal of Molecular Medicine 10.5 (2002): 593-597.
Chicago
Nagai, N., Oshita, T., Mukai, K., Shiroyama, Y., Shigemasa, K., Ohama, K."GnRH agonist inhibits human telomerase reverse transcriptase mRNA expression in endometrial cancer cells". International Journal of Molecular Medicine 10, no. 5 (2002): 593-597. https://doi.org/10.3892/ijmm.10.5.593