Detection of circulating prostate tumor cells: alternative spliced variant of PSM induced false-positive result

Hisatomi,H.; Nagao,K.; Kawakita,M.; Matsuda,T.; Hirata,H.; Yamamoto,S.; Nakamoto,T.; Harasawa,H.; Kaneko,N.; Hikiji,K.; Tsukada,Y.

doi:10.3892/ijmm.10.5.619

November 2002 Volume 10 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

November 2002 Volume 10 Issue 5

Full Size Image

Article

Detection of circulating prostate tumor cells: alternative spliced variant of PSM induced false-positive result

Authors:
- H. Hisatomi
- K. Nagao
- M. Kawakita
- T. Matsuda
- H. Hirata
- S. Yamamoto
- T. Nakamoto
- H. Harasawa
- N. Kaneko
- K. Hikiji
- Y. Tsukada
View Affiliations / Copyright

Affiliations: Center for Molecular Biology and Cytogenetics, SRL, Inc., Hino, Tokyo 191-0002, Japan. hisatomi@srl.srl-inc.co.jp
Pages: 619-622
|
Published online on: November 1, 2002

https://doi.org/10.3892/ijmm.10.5.619
Expand metrics +

Abstract

RT-nested PCR has been introduced as a highly specific and sensitive assay method to detect the prostate-specific membrane antigen (PSM) mRNA in peripheral blood. However, appreciable percentages of false-positive cases have been reported. Additionally, primer sets reported previously could not discriminate between PSM and PSM', an alternatively spliced variant, mRNA. These isoforms can be produced from a single gene. Switches in alternative splicing patterns are often controlled with strict cell-type or developmental-stage specificity. Therefore, it is most important to discriminate between PSM mRNA and PSM' mRNA. Using our highly specific primer sets, PSM mRNA was detected in 3 of 24 peripheral blood samples of normal male volunteers (12.5%) and was not detected in peripheral blood of 11 normal female volunteers. PSM' mRNA was detected in 5 of 24 peripheral blood samples of normal male volunteers (20.8%) and in 4 of 11 of normal female volunteers (36.4%). PSM' mRNA induced false-positive results, it is important for genetic diagnosis of prostate cancer to discriminate between PSM and PSM' using our primer sets with high specificity. The advances in the uniquely designed primer sets may allow researchers to detect a real PSM mRNA without PSM' mRNA.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection of circulating prostate tumor cells: alternative spliced variant of PSM induced false-positive result

This article is mentioned in:

Abstract

Related Articles