EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor

  • Authors:
    • Maria Haagg
    • Asa Liljegren
    • Jorgen Carlsson
    • Lars Ronnstrand
    • Johan Lennartsson
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijmm.10.5.655
  • Pages: 655-659
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Abstract

Dextran-conjugated EGF (EGF-dextran) has a potential use for targeted radionuclide therapy of tumors that overexpress the epidermal growth factor receptor (EGFR). There are plans to treat both bladder carcinomas and malignant gliomas with local injections of radiolabeled EGF-dextran since these tumors often express high levels of EGFR. In this report we show that EGF and EGF-dextran differentially activate the EGFR. In the human glioma cell line U-343, activation of the serine/threonine kinases Erk and Akt is identical upon stimulation with EGF or EGF-dextran. However, the effect on phospholipase Cγ1 (PLCγ1) phosphorylation differs. In cells stimulated with EGF-dextran, the PLCγ1 phosphorylation is lower than in cells stimulated with EGF. This observation could be explained by the fact that the PLCγ1 association sites in the EGFR, tyrosine residues 992 and 1173, were phosphorylated to a lower degree when the receptor was stimulated with EGF-dextran as compared to with EGF.

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November 2002
Volume 10 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Haagg M, Liljegren A, Carlsson J, Ronnstrand L and Lennartsson J: EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor. Int J Mol Med 10: 655-659, 2002.
APA
Haagg, M., Liljegren, A., Carlsson, J., Ronnstrand, L., & Lennartsson, J. (2002). EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor. International Journal of Molecular Medicine, 10, 655-659. https://doi.org/10.3892/ijmm.10.5.655
MLA
Haagg, M., Liljegren, A., Carlsson, J., Ronnstrand, L., Lennartsson, J."EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor". International Journal of Molecular Medicine 10.5 (2002): 655-659.
Chicago
Haagg, M., Liljegren, A., Carlsson, J., Ronnstrand, L., Lennartsson, J."EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor". International Journal of Molecular Medicine 10, no. 5 (2002): 655-659. https://doi.org/10.3892/ijmm.10.5.655