Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver

  • Authors:
    • Ryoko Kuromatsu
    • Masatoshi Tanaka
    • Yoshihiro Shimauchi
    • Riko Harada
    • Eiji Ando
    • Satoshi Itano
    • Ryukichi Kumashiro
    • Shuichi Fukuda
    • Koji Okuda
    • Michio Sata
  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijmm.11.2.199
  • Pages: 199-204
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Abstract

Radiofrequency ablation (RFA) is an effective procedure for localized hepatocellular carcinoma. Contrast-enhanced CT depicts the ablated area as a hypoattenuated area without hepatic blood flow; however, light microscopy does not show obvious necrosis in the ablated area. We evaluated liver tissue changes after RFA by light microscopy and electron microscopy. The normal livers of three anesthetized pigs were coagulated using RFA after laparotomy. The liver was examined immediately, and 1 week after operation by light and electron microscopy. After RFA, the liver parenchyma surrounding the needle electrode was brown in color and surrounded by a red marginal zone separate from the normal liver parenchyma. Hematoxylin-eosin staining of the central area did not show cell necrosis, and the structures of liver sinusoids, liver cell cord and the nuclei of hepatocytes were preserved. However, electron microscopic examination of tissue immediately after RFA showed destruction of mitochondria of hepatocytes and fixation of sinusoidal cells. One week later, there was a large quantity of debris in the enlarged sinusoids, in addition to irreversible destruction of hepatocyte organelles. RFA of the porcine liver causes hepatocyte damage. This damage was not evident by light microscopy but clearly identified by electron microscopy.

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February 2003
Volume 11 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kuromatsu R, Tanaka M, Shimauchi Y, Harada R, Ando E, Itano S, Kumashiro R, Fukuda S, Okuda K, Sata M, Sata M, et al: Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver. Int J Mol Med 11: 199-204, 2003.
APA
Kuromatsu, R., Tanaka, M., Shimauchi, Y., Harada, R., Ando, E., Itano, S. ... Sata, M. (2003). Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver. International Journal of Molecular Medicine, 11, 199-204. https://doi.org/10.3892/ijmm.11.2.199
MLA
Kuromatsu, R., Tanaka, M., Shimauchi, Y., Harada, R., Ando, E., Itano, S., Kumashiro, R., Fukuda, S., Okuda, K., Sata, M."Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver". International Journal of Molecular Medicine 11.2 (2003): 199-204.
Chicago
Kuromatsu, R., Tanaka, M., Shimauchi, Y., Harada, R., Ando, E., Itano, S., Kumashiro, R., Fukuda, S., Okuda, K., Sata, M."Light and electron microscopic analyses of immediate and late tissue damage caused by radiofrequency ablation in porcine liver". International Journal of Molecular Medicine 11, no. 2 (2003): 199-204. https://doi.org/10.3892/ijmm.11.2.199