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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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September 2003 Volume 12 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2003 Volume 12 Issue 3

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Article

Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes

  • Authors:
    • Hong Li
    • Yuan Sun
    • Qing-You Kong
    • Kai-Li Zhang
    • Xiao-Wei Wang
    • Xiao-Yan Chen
    • Qian Wang
    • Jia Liu
  • View Affiliations / Copyright

    Affiliations: Cancer Institute and Laboratory of Cell Biology and Molecular Genetics, College of Basic Medical Sciences, Dalian Medical University, 116027 Dalian, P.R. China. lihongmcn@yahoo.com.cn
  • Pages: 299-304
    |
    Published online on: September 1, 2003
       https://doi.org/10.3892/ijmm.12.3.299
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Abstract

Precise dissection of defined histological regions for nucleic acid and protein isolation is a precedent step in finding out cancer-related alterations, and high quality tissue microarrays are demanded in the validation of screened genetic alterations by multiple in situ approaches. In this study, a combined technique was developed by which sample isolation and tissue array construction could be performed on the defined morphological region(s) in single tissue block. The RNA and protein samples generated from the selected portions were of good quality and sufficient for multiple experimental purposes. The frozen tissue arrays constructed on a novel recipient are suitable for multiple in situ evaluations including immunohistochemical staining and mRNA hybridisation. In most cases, the data obtained from in situ assays coincided well with the ones revealed by RT-PCR and Western blot hybridisation. The potential experimental bias caused by cell contamination can be amended by tissue array-based retrospective examination. The combination of tissue-selective sample preparations with tissue array construction thus provide a tool by which comprehensive cancer research can be performed on defined histological regions in a series of single frozen tissue blocks.

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Copy and paste a formatted citation
Spandidos Publications style
Li H, Sun Y, Kong Q, Zhang K, Wang X, Chen X, Wang Q and Liu J: Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes. Int J Mol Med 12: 299-304, 2003.
APA
Li, H., Sun, Y., Kong, Q., Zhang, K., Wang, X., Chen, X. ... Liu, J. (2003). Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes. International Journal of Molecular Medicine, 12, 299-304. https://doi.org/10.3892/ijmm.12.3.299
MLA
Li, H., Sun, Y., Kong, Q., Zhang, K., Wang, X., Chen, X., Wang, Q., Liu, J."Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes". International Journal of Molecular Medicine 12.3 (2003): 299-304.
Chicago
Li, H., Sun, Y., Kong, Q., Zhang, K., Wang, X., Chen, X., Wang, Q., Liu, J."Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes". International Journal of Molecular Medicine 12, no. 3 (2003): 299-304. https://doi.org/10.3892/ijmm.12.3.299
Copy and paste a formatted citation
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Spandidos Publications style
Li H, Sun Y, Kong Q, Zhang K, Wang X, Chen X, Wang Q and Liu J: Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes. Int J Mol Med 12: 299-304, 2003.
APA
Li, H., Sun, Y., Kong, Q., Zhang, K., Wang, X., Chen, X. ... Liu, J. (2003). Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes. International Journal of Molecular Medicine, 12, 299-304. https://doi.org/10.3892/ijmm.12.3.299
MLA
Li, H., Sun, Y., Kong, Q., Zhang, K., Wang, X., Chen, X., Wang, Q., Liu, J."Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes". International Journal of Molecular Medicine 12.3 (2003): 299-304.
Chicago
Li, H., Sun, Y., Kong, Q., Zhang, K., Wang, X., Chen, X., Wang, Q., Liu, J."Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes". International Journal of Molecular Medicine 12, no. 3 (2003): 299-304. https://doi.org/10.3892/ijmm.12.3.299
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