Thrombin stimulates IL-6 and IL-8 expression in cytomegalovirus-infected human retinal pigment epithelial cells

  • Authors:
    • Martin Scholz
    • Jens-Uwe Vogel
    • Gerold Höver
    • Ruslan Kotchetkov
    • Jaroslav Cinatl
    • Hans Wilhelm Doerr
    • Jindrich Cinatl
  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/ijmm.13.2.327
  • Pages: 327-331
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Abstract

Recently, we reported that thrombin specifically stimulates protease-activated receptor-1 (PAR-1) signaling in RPE entailing inhibition of Sp1 dependent HCMV replication. We now studied whether thrombin modulates the expression of the proinflammatory cytokine/chemokines IL-6 and IL-8 in mock- and cytomegalovirus-infected human retinal pigment epithelial cells (RPE). Our data show that thrombin/PAR-1 stimulates IL-6 and IL-8 gene transcription and protein secretion in both mock- and HCMV-infected RPE. Thrombin/PAR-1-mediated signaling stimulated PKC and NF-κB-dependent IL-6 and IL-8 gene expression via phosphoinositide 3-kinase and further downstream via p42/44 and p38 MAPKs. Thus, thrombin/PAR-1-mediated IL-6/IL-8 gene expression is uncoupled from Sp1 inhibition and may support proinflammatory pathomechanisms probably involved in hemorrhage/HCMV retinitis progression.

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February 2004
Volume 13 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Scholz, M., Vogel, J., Höver, G., Kotchetkov, R., Cinatl, J., Doerr, H.W., & Cinatl, J. (2004). Thrombin stimulates IL-6 and IL-8 expression in cytomegalovirus-infected human retinal pigment epithelial cells. International Journal of Molecular Medicine, 13, 327-331. https://doi.org/10.3892/ijmm.13.2.327
MLA
Scholz, M., Vogel, J., Höver, G., Kotchetkov, R., Cinatl, J., Doerr, H. W., Cinatl, J."Thrombin stimulates IL-6 and IL-8 expression in cytomegalovirus-infected human retinal pigment epithelial cells". International Journal of Molecular Medicine 13.2 (2004): 327-331.
Chicago
Scholz, M., Vogel, J., Höver, G., Kotchetkov, R., Cinatl, J., Doerr, H. W., Cinatl, J."Thrombin stimulates IL-6 and IL-8 expression in cytomegalovirus-infected human retinal pigment epithelial cells". International Journal of Molecular Medicine 13, no. 2 (2004): 327-331. https://doi.org/10.3892/ijmm.13.2.327