Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization

  • Authors:
    • Yasuhiro Ogawa
    • Toshihiro Kobayashi
    • Akihito Nishioka
    • Shinji Kariya
    • Takenao Ohnishi
    • Shinji Hamasato
    • Harumichi Seguchi
    • Shoji Yoshida
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijmm.13.3.383
  • Pages: 383-388
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Abstract

In our previous study, we examined the effect of exogenous hydrogen peroxide, which causes a potent oxidative stress and has been demonstrated to be a potent apoptosis-inducer in many kinds of cells. We found that the addition of 1 or 10 mM hydrogen peroxide induced reactive oxygen species (ROS) formation, oxidative DNA damage, dysfunction of the mitochondrial membrane potential, and early apoptotic changes in the human osteosarcoma cell line HS-Os-1. We therefore concluded that intracellular ROS formation was involved in the hydrogen peroxide-induced apoptosis of HS-Os-1 cells. In contrast to the osteosarcoma cell line HS-Os-1, human peripheral T cells are considered to be easily susceptible to oxidative stress, because these cells lack peroxidase activity. Therefore, in this study, we investigated the site of ROS formation by utilizing MitoCapture, H2DCFDA (succinimidyl ester of dichloro-dihydrofluorescein diacetate), DAPI (4',6-diamidino-2-phenylindole), and LysoSensor. Our results showed that ROS formation was apparently diffusely distributed in T cells oxidatively stressed with 0.1 mM hydrogen peroxide. Moreover, lysosomal swelling and deformity, possibly revealing lysosomal membrane destabilization, were observed in these cells. Based on the above results, there exists an apoptotic cascade involving early lysosomal membrane destabilization in the hydrogen peroxide-induced apoptosis of human peripheral T cells. Therefore, the possible involvement of lysosomal protease leakage caused by hydroxyl radical formation in lysosomes (possibly resulting in mitochondrial membrane dysfunction) is considered to play an important role in hydrogen peroxide-induced T cell apoptosis.

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March 2004
Volume 13 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ogawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H and Yoshida S: Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization. Int J Mol Med 13: 383-388, 2004.
APA
Ogawa, Y., Kobayashi, T., Nishioka, A., Kariya, S., Ohnishi, T., Hamasato, S. ... Yoshida, S. (2004). Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization. International Journal of Molecular Medicine, 13, 383-388. https://doi.org/10.3892/ijmm.13.3.383
MLA
Ogawa, Y., Kobayashi, T., Nishioka, A., Kariya, S., Ohnishi, T., Hamasato, S., Seguchi, H., Yoshida, S."Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization". International Journal of Molecular Medicine 13.3 (2004): 383-388.
Chicago
Ogawa, Y., Kobayashi, T., Nishioka, A., Kariya, S., Ohnishi, T., Hamasato, S., Seguchi, H., Yoshida, S."Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization". International Journal of Molecular Medicine 13, no. 3 (2004): 383-388. https://doi.org/10.3892/ijmm.13.3.383