Mesenchymal stem cells contribute to the renal repair of acute tubular epithelial injury

  • Authors:
    • Maria Beatriz Herrera
    • Benedetta Bussolati
    • Stefania Bruno
    • Valentina Fonsato
    • Giuseppe Mauriello Romanazzi
    • Giovanni Camussi
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1035
  • Pages: 1035-1041
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Acute renal failure (ARF) is a common disease with high morbidity and mortality. Recovery from ARF is dependent on the replacement of necrotic tubular cells with functional tubular epithelium. Recent advancement in developmental biology led to the discovery of immature mesenchymal stem cells (MSCs) in bone marrow and several established organs and to the definition of their potential in the recovery from tissue injury. We investigated the effect of MSCs infusion on the recovery from ARF induced by intramuscle injection of glycerol in C57/BL6 mice. In this model, ARF is associated with an extensive necrosis of tubular epithelial cells due to myoglobin- and hemoglobin-induced injury. MSCs were obtained from bone marrow of transgenic mice expressing green fluorescent protein (GFP). MSC GFP-positive cells (MSC-GFP+) injected intravenously homed to the kidney of mice with glycerol-induced ARF but not to the kidney of normal mice. MSC-GFP+ localized in the context of the tubular epithelial lining and expressed cytokeratin, indicating that MSCs engrafted in the damaged kidney, differentiated into tubular epithelial cells and promoted the recovery of morphological and functional alterations. Moreover, MSCs enhanced tubular proliferation as detected by the increased number of proliferating cell nuclear antigen (PCNA) positive cells. A significant contribution of the engrafted MSCs in the regeneration of tubular epithelial cells was shown by the presence of a consistent number of GFP+ tubular cells 21 days after the induction of injury. In conclusion, these results indicated a tropism of MSCs for the injured kidney and a potential contribution of these cells to tubular regeneration and to the recovery from ARF.

Related Articles

Journal Cover

December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Herrera MB, Bussolati B, Bruno S, Fonsato V, Romanazzi GM and Camussi G: Mesenchymal stem cells contribute to the renal repair of acute tubular epithelial injury. Int J Mol Med 14: 1035-1041, 2004
APA
Herrera, M.B., Bussolati, B., Bruno, S., Fonsato, V., Romanazzi, G.M., & Camussi, G. (2004). Mesenchymal stem cells contribute to the renal repair of acute tubular epithelial injury. International Journal of Molecular Medicine, 14, 1035-1041. https://doi.org/10.3892/ijmm.14.6.1035
MLA
Herrera, M. B., Bussolati, B., Bruno, S., Fonsato, V., Romanazzi, G. M., Camussi, G."Mesenchymal stem cells contribute to the renal repair of acute tubular epithelial injury". International Journal of Molecular Medicine 14.6 (2004): 1035-1041.
Chicago
Herrera, M. B., Bussolati, B., Bruno, S., Fonsato, V., Romanazzi, G. M., Camussi, G."Mesenchymal stem cells contribute to the renal repair of acute tubular epithelial injury". International Journal of Molecular Medicine 14, no. 6 (2004): 1035-1041. https://doi.org/10.3892/ijmm.14.6.1035