Generation and characterization of HIV-1 clones chimeric for subtypes B and C nef

  • Authors:
    • Abhay Jere
    • Ahmad Piroozmand
    • Srikanth Tripathy
    • Ramesh Paranjape
    • Akiko Sakurai
    • Mikako Fujita
    • Akio Adachi
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1087
  • Pages: 1087-1090
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Abstract

The impact of human immunodeficiency virus type 1 (HIV-1) Nef on viral infectivity was evaluated by characterization of chimeric clones. Chimera with respect to the nef gene were constructed between subtypes B and C, and monitored for their replication in human peripheral blood mononuclear cells. The parental clones used were pNL432 (subtype B) and pIndie-C1 (Indian subtype C), which show considerable sequence heterogeneity in nef and distinct viral growth phenotype. While an enhancing effect of Nef on viral infectivity was noted, no significant growth difference was observed between the parental and chimeric clones. The difference in growth potential of the two subtype clones was mainly ascribable to viral sequence(s) other than nef. Our results here clearly showed that HIV-1 Nef does not significantly affect the in vitro viral infectivity in natural target cells.

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December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jere A, Piroozmand A, Tripathy S, Paranjape R, Sakurai A, Fujita M and Adachi A: Generation and characterization of HIV-1 clones chimeric for subtypes B and C nef. Int J Mol Med 14: 1087-1090, 2004.
APA
Jere, A., Piroozmand, A., Tripathy, S., Paranjape, R., Sakurai, A., Fujita, M., & Adachi, A. (2004). Generation and characterization of HIV-1 clones chimeric for subtypes B and C nef. International Journal of Molecular Medicine, 14, 1087-1090. https://doi.org/10.3892/ijmm.14.6.1087
MLA
Jere, A., Piroozmand, A., Tripathy, S., Paranjape, R., Sakurai, A., Fujita, M., Adachi, A."Generation and characterization of HIV-1 clones chimeric for subtypes B and C nef". International Journal of Molecular Medicine 14.6 (2004): 1087-1090.
Chicago
Jere, A., Piroozmand, A., Tripathy, S., Paranjape, R., Sakurai, A., Fujita, M., Adachi, A."Generation and characterization of HIV-1 clones chimeric for subtypes B and C nef". International Journal of Molecular Medicine 14, no. 6 (2004): 1087-1090. https://doi.org/10.3892/ijmm.14.6.1087