Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis

  • Authors:
    • Hui Wang
    • Zuomin Zhou
    • Li Lu
    • Zhiyang Xu
    • Jiahao Sha
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/ijmm.15.6.949
  • Pages: 949-953
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Abstract

Using cDNA microarray hybridization from a human testicular cDNA library, one gene named lactate dehydrogenase A-like gene (LDHL, also known as LDHL6B) was cloned. LDHL exhibited 3.8-fold difference at expression level between adult and fetal human testes. The full cDNA length of LDHL is 1680 bp and had a 1145 bp open reading frame, which encoded a 41.9 kDa protein of 381 amino acids. Sequence analysis showed that LDHL harbors all the domains (one lactate/malate dehydrogenase, NAD binding domain and one lactate/malate dehydrogenase, α/β C-terminal domain) in lactate dehydrogenase gene family. Blasting human genome database localized LDHL to human chromosome 15q22.2 and it was an intronless gene. Results of multiple-tissue PCR and real-time PCR showed that LDHL expressed mainly in testis and its mRNA abundance was testis development- related. In summary, LDHL is believed to be involved in testis development and spermatogenesis.

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June 2005
Volume 15 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang H, Zhou Z, Lu L, Xu Z and Sha J: Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis. Int J Mol Med 15: 949-953, 2005
APA
Wang, H., Zhou, Z., Lu, L., Xu, Z., & Sha, J. (2005). Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis. International Journal of Molecular Medicine, 15, 949-953. https://doi.org/10.3892/ijmm.15.6.949
MLA
Wang, H., Zhou, Z., Lu, L., Xu, Z., Sha, J."Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis". International Journal of Molecular Medicine 15.6 (2005): 949-953.
Chicago
Wang, H., Zhou, Z., Lu, L., Xu, Z., Sha, J."Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis". International Journal of Molecular Medicine 15, no. 6 (2005): 949-953. https://doi.org/10.3892/ijmm.15.6.949