GLP-1 signalling and effects on glucose metabolism in myocytes from type 2 diabetic patients

  • Authors:
    • Nieves González
    • Verónica Sancho
    • Antonio Martín-Duce
    • Pilar Tornero-Esteban
    • Isabel Valverde
    • Willy J. Malaisse
    • María L. Villanueva-Pe?acarrillo
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijmm.16.4.747
  • Pages: 747-752
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Abstract

Changes in the activity of glycogen synthase a and related kinases (phosphatidylinositol-3-kinase, protein kinase B, p44/42 MAP kinases and p70s6 kinase) evoked by GLP-1 in human myocytes from normal subjects were recently implied in the effect of this hormone upon D-glucose transport and glycogen synthesis in the same cells. The major aims of the present study were i) to investigate the possible extension of this knowledge to myocytes obtained from type 2 diabetic patients, ii) to compare in these patients the response to GLP-1, insulin or the structurally related GLP-1 peptides, exendin (1-39)amide and exendin(9-39)amide, and iii) to explore possible differences in the responsiveness to these agents between normal and diabetic subjects. Apart from the much higher basal PI3K activity and impaired response to insulin of p44/42 MAP kinases in the diabetic patients, the changes in enzyme activity caused by either hormone or peptide, although not identical, were essentially comparable. Nevertheless, significant differences in glucose transport and metabolism parameters were observed in the diabetic patients vs. normal subjects: in the diabetic patients, basal 2-deoxy-glucose uptake and glycogen synthase a activity were lower, accompanied by a similar increasing effect of GLP-1 or insulin; yet, the basal value for glycogen synthesis was higher, coinciding with a lesser relative increment in response to GLP-1 or insulin.

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October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
González N, Sancho V, Martín-Duce A, Tornero-Esteban P, Valverde I, Malaisse WJ and Villanueva-Pe?acarrillo ML: GLP-1 signalling and effects on glucose metabolism in myocytes from type 2 diabetic patients. Int J Mol Med 16: 747-752, 2005
APA
González, N., Sancho, V., Martín-Duce, A., Tornero-Esteban, P., Valverde, I., Malaisse, W.J., & Villanueva-Pe?acarrillo, M.L. (2005). GLP-1 signalling and effects on glucose metabolism in myocytes from type 2 diabetic patients. International Journal of Molecular Medicine, 16, 747-752. https://doi.org/10.3892/ijmm.16.4.747
MLA
González, N., Sancho, V., Martín-Duce, A., Tornero-Esteban, P., Valverde, I., Malaisse, W. J., Villanueva-Pe?acarrillo, M. L."GLP-1 signalling and effects on glucose metabolism in myocytes from type 2 diabetic patients". International Journal of Molecular Medicine 16.4 (2005): 747-752.
Chicago
González, N., Sancho, V., Martín-Duce, A., Tornero-Esteban, P., Valverde, I., Malaisse, W. J., Villanueva-Pe?acarrillo, M. L."GLP-1 signalling and effects on glucose metabolism in myocytes from type 2 diabetic patients". International Journal of Molecular Medicine 16, no. 4 (2005): 747-752. https://doi.org/10.3892/ijmm.16.4.747