Reverse transcription-polymerase chain reaction in situ hybridization for SYT-SSX fusion gene transcripts in synovial sarcomas

  • Authors:
    • Shigeto Kawauchi
    • Toshiro Fukuda
    • Yasuyo Chochi
    • Tomoko Kondo
    • Atsunori Oga
    • Kohsuke Sasaki
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/ijmm.16.4.763
  • Pages: 763-766
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Abstract

More than 90% of synovial sarcomas (SSs) possess a non-random chromosomal translocation t(X;18)(p11;q11) and express SYT-SSX fusion gene products originating from the translocation. To test whether it is possible to detect the SYT-SSX mRNA on archival formalin-fixed paraffin-embedded SS tissue sections using PCR-based in situ amplification technique, we performed reverse transcription-polymerase chain reaction in situ hybridization (RT-PCR ISH) for the SYT-SSX fusion gene mRNA. In three types of SSs, monophasic fibrous, biphasic and poorly differentiated, NBT/BCIP signals corresponding to SYT-SSX mRNA were uniformly and predominantly positive in the sarcoma cell cytoplasm. Our results indicated that SS cells uniformly possessed the SYT-SSX fusion genes and expressed their transcripts. Furthermore, our results were thought to support monoclonal origin of epithelial and spindle cell components of biphasic SSs. Thus, specific chromosomal translocation t(X;18) is likely to be an early event in the development of SSs, and the expression of SYT-SSX fusion gene products is thought to be crucial for the tumorigenesis of SSs.

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October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kawauchi S, Fukuda T, Chochi Y, Kondo T, Oga A and Sasaki K: Reverse transcription-polymerase chain reaction in situ hybridization for SYT-SSX fusion gene transcripts in synovial sarcomas. Int J Mol Med 16: 763-766, 2005
APA
Kawauchi, S., Fukuda, T., Chochi, Y., Kondo, T., Oga, A., & Sasaki, K. (2005). Reverse transcription-polymerase chain reaction in situ hybridization for SYT-SSX fusion gene transcripts in synovial sarcomas. International Journal of Molecular Medicine, 16, 763-766. https://doi.org/10.3892/ijmm.16.4.763
MLA
Kawauchi, S., Fukuda, T., Chochi, Y., Kondo, T., Oga, A., Sasaki, K."Reverse transcription-polymerase chain reaction in situ hybridization for SYT-SSX fusion gene transcripts in synovial sarcomas". International Journal of Molecular Medicine 16.4 (2005): 763-766.
Chicago
Kawauchi, S., Fukuda, T., Chochi, Y., Kondo, T., Oga, A., Sasaki, K."Reverse transcription-polymerase chain reaction in situ hybridization for SYT-SSX fusion gene transcripts in synovial sarcomas". International Journal of Molecular Medicine 16, no. 4 (2005): 763-766. https://doi.org/10.3892/ijmm.16.4.763