VP-16 (etoposide) and calphostin C trigger different nuclear but akin cytoplasmic patterns of changes in the distribution and activity of protein kinase C-βI in polyomavirus-transformed pyF111 rat fibroblasts

  • Authors:
    • Anna Chiarini
    • James F. Whitfield
    • Ubaldo Armato
    • Ilaria Dal Pra
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/ijmm.17.1.111
  • Pages: 111-120
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Abstract

Protein kinase C (PKC) isoforms regulate cell proliferation and apoptosis. Since the PKC isoenzyme complement varies considerably from cell type to cell type, a PKC's responsiveness to an apoptogenic challenge must be defined for both the type of apoptogen and the type of cell. We have already reported that the changes in the distribution and activity of PKC-δ in apoptosing polyomavirus-infected/transformed Fischer rat embryo pyF111 fibroblasts depend on the type of apoptogen. Here, we show that this is also true for PKC-βI in pyF111 cells treated with the slow DNA-damaging VP-16 (etoposide) or the fast-acting (in the cytoplasm) calphostin C. These apoptogens caused quite different shifts of the PKC-βI level and activity in the nuclear membrane (NM) and nucleoplasm (NP), but corresponding changes in the cytosol (CS) and cytoplasmic particulate (CP) fractions. The hefty translocation of PKC-βI onto the CP fraction and its increased activity there suggest the possible triggering of a cytochrome c/caspase-mediated apoptosis-inducing mechanism common to both agents. The present results are a necessary lead-up to functional proteomic analyses aimed at identifying the molecules forming the local PKC-βI signalling modules under different conditions.

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January 2006
Volume 17 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chiarini A, Whitfield JF, Armato U and Dal Pra I: VP-16 (etoposide) and calphostin C trigger different nuclear but akin cytoplasmic patterns of changes in the distribution and activity of protein kinase C-βI in polyomavirus-transformed pyF111 rat fibroblasts. Int J Mol Med 17: 111-120, 2006.
APA
Chiarini, A., Whitfield, J.F., Armato, U., & Dal Pra, I. (2006). VP-16 (etoposide) and calphostin C trigger different nuclear but akin cytoplasmic patterns of changes in the distribution and activity of protein kinase C-βI in polyomavirus-transformed pyF111 rat fibroblasts. International Journal of Molecular Medicine, 17, 111-120. https://doi.org/10.3892/ijmm.17.1.111
MLA
Chiarini, A., Whitfield, J. F., Armato, U., Dal Pra, I."VP-16 (etoposide) and calphostin C trigger different nuclear but akin cytoplasmic patterns of changes in the distribution and activity of protein kinase C-βI in polyomavirus-transformed pyF111 rat fibroblasts". International Journal of Molecular Medicine 17.1 (2006): 111-120.
Chicago
Chiarini, A., Whitfield, J. F., Armato, U., Dal Pra, I."VP-16 (etoposide) and calphostin C trigger different nuclear but akin cytoplasmic patterns of changes in the distribution and activity of protein kinase C-βI in polyomavirus-transformed pyF111 rat fibroblasts". International Journal of Molecular Medicine 17, no. 1 (2006): 111-120. https://doi.org/10.3892/ijmm.17.1.111