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Mutagenicity of microcystin-LR in human RSa cells.

  • Authors:
    • H Suzuki
    • M F Watanabe
    • Y Wu
    • T Sugita
    • K Kita
    • T Sato
    • X Wang
    • H Tanzawa
    • S Sekiya
    • N Suzuki

  • View Affiliations

  • Published online on: July 1, 1998     https://doi.org/10.3892/ijmm.2.1.109
  • Pages: 109-121
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Abstract

Microcystin-LR (MCLR) is a potent cyclic heptapeptidic hepatotoxin produced by bloom-forming cyanobacteria. The mutagenicity induced by this compound in cultured human RSa cells was found by determination of ouabain-resistant (OuaR) mutation, with the highest frequency at the concentration of 15 microgram/ml. Moreover, base substitution mutations at K-ras codon 12 in genomic DNA, assessed by polymerase chain reaction (PCR) and differential dot-blot hybridization using digoxigenin-labeled probes, were detected in RSa cells 6 days after exposure to MCLR (7.5-15 microgram/ml).

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Jul 1998
Volume 2 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Suzuki H, Watanabe M, Wu Y, Sugita T, Kita K, Sato T, Wang X, Tanzawa H, Sekiya S, Suzuki N, Suzuki N, et al: Mutagenicity of microcystin-LR in human RSa cells.. Int J Mol Med 2: 109-121, 1998
APA
Suzuki, H., Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T. ... Suzuki, N. (1998). Mutagenicity of microcystin-LR in human RSa cells.. International Journal of Molecular Medicine, 2, 109-121. https://doi.org/10.3892/ijmm.2.1.109
MLA
Suzuki, H., Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S., Suzuki, N."Mutagenicity of microcystin-LR in human RSa cells.". International Journal of Molecular Medicine 2.1 (1998): 109-121.
Chicago
Suzuki, H., Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S., Suzuki, N."Mutagenicity of microcystin-LR in human RSa cells.". International Journal of Molecular Medicine 2, no. 1 (1998): 109-121. https://doi.org/10.3892/ijmm.2.1.109