Radiation kills human peripheral T cells by a Fas-independent mechanism.

  • Authors:
    • Y Ogawa
    • A Nishioka
    • T Inomata
    • S Yoshida
    • K I Nakayama
    • S Kataoka
  • View Affiliations

  • Published online on: October 1, 1998     https://doi.org/10.3892/ijmm.2.4.403
  • Pages: 403-411
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Abstract

The mechanism by which radiation induces human peripheral T cell apoptosis is not known. We examined sequential changes in post-irradiated peripheral blood mononuclear cells (PBMC(S)) taken from normal volunteers, by using flow-cytometer and an anti-CD3 monoclonal antibody, annexin V, propidium iodide, anti-Fas antibody, and anti-Fas ligand antibody. After 5 or 10 Gy of irradiation with a 60Co radiation therapy unit, most of the human peripheral T cells showed positivity against annexin V in 15 h, and positivity against propidium iodide in 23 h after irradiation. On a microscopy-video system, approximately 80% of mononuclear cells revealed apoptotic changes in 24 h after irradiation. Because of its proposed role in activation-induced cytotoxicity, we also examined the Fas (CD95/Apo-1) pathway in killing T cells by irradiation. Irradiated PBMC, displayed no increase in surface Fas expression and caspase-3 activity relative to non-irradiated cells. In addition, the anti-Fas ligand failed to eliminate the apoptotic death of PBMC, after irradiation. These results suggest that irradiation induces direct apoptosis of T cells by a Fas-independent mechanism.

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Oct 1998
Volume 2 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ogawa Y, Nishioka A, Inomata T, Yoshida S, Nakayama K and Kataoka S: Radiation kills human peripheral T cells by a Fas-independent mechanism.. Int J Mol Med 2: 403-411, 1998.
APA
Ogawa, Y., Nishioka, A., Inomata, T., Yoshida, S., Nakayama, K., & Kataoka, S. (1998). Radiation kills human peripheral T cells by a Fas-independent mechanism.. International Journal of Molecular Medicine, 2, 403-411. https://doi.org/10.3892/ijmm.2.4.403
MLA
Ogawa, Y., Nishioka, A., Inomata, T., Yoshida, S., Nakayama, K., Kataoka, S."Radiation kills human peripheral T cells by a Fas-independent mechanism.". International Journal of Molecular Medicine 2.4 (1998): 403-411.
Chicago
Ogawa, Y., Nishioka, A., Inomata, T., Yoshida, S., Nakayama, K., Kataoka, S."Radiation kills human peripheral T cells by a Fas-independent mechanism.". International Journal of Molecular Medicine 2, no. 4 (1998): 403-411. https://doi.org/10.3892/ijmm.2.4.403