Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line

  • Authors:
    • Eun-Kyung Kim
    • Kang-Beom Kwon
    • Mi-Jeong Han
    • Mi-Young Song
    • Ji-Hyun Lee
    • Youn-Suk Ko
    • Byung-Cheal Shin
    • Jiahua Yu
    • Young-Rae Lee
    • Do-Gon Ryu
    • Jin-Woo Park
    • Byung-Hyun Park
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/ijmm.20.1.123
  • Pages: 123-128
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Abstract

Scutellaria barbata has been used to treat cancer in Chinese medicine. The responsible anticancer mechanism, however, is not clear. Here we demonstrated an inhibitory mechanism due to a Scutellaria barbata extract (SBE) on a human promyelocytic leukemia cell line (HL-60) that has a mutation in the tumor suppressor gene p53. HL-60 cells were incubated with various concentrations of SBE. After a 24-h incubation, cytotoxicity and apoptosis were determined by MTT and DNA fragmentation assay, respectively. After treatment with SBE, cell cycle arrest was determined by measuring the cell number stained by 5'-bromo-2'-deoxyuridine (BrdU) and 7-amino-actinomycin D (7-AAD). Treatment of cells with SBE resulted in a concentration- and time-dependent inhibition of growth and a G1 phase arrest of the cell cycle. This effect was associated with a marked decrease in the protein expression of cyclin A, D1, D2, D3, and E and their activating partners, cyclin-dependent kinases (CDK) 2, 4, and 6 with concomitant upregulation of p21, cyclin-dependent kinase inhibitor. Downstream of the CDK inhibitory protein-CDK/cyclin cascade, SBE decreased phosphorylation level of retinoblastoma protein. SBE treatment also resulted in apoptosis evidenced by an increase of sub-G1 phase cells, DNA fragmentation and degradation of the inhibitory protein for the caspase-activated deoxyribonuclease. The molecular mechanism during SBE-mediated growth inhibition in HL-60 cells may be due to modulation of the cell-cycle machinery and the induction of apoptosis.

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July 2007
Volume 20 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Kim, E., Kwon, K., Han, M., Song, M., Lee, J., Ko, Y. ... Park, B. (2007). Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line. International Journal of Molecular Medicine, 20, 123-128. https://doi.org/10.3892/ijmm.20.1.123
MLA
Kim, E., Kwon, K., Han, M., Song, M., Lee, J., Ko, Y., Shin, B., Yu, J., Lee, Y., Ryu, D., Park, J., Park, B."Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line". International Journal of Molecular Medicine 20.1 (2007): 123-128.
Chicago
Kim, E., Kwon, K., Han, M., Song, M., Lee, J., Ko, Y., Shin, B., Yu, J., Lee, Y., Ryu, D., Park, J., Park, B."Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line". International Journal of Molecular Medicine 20, no. 1 (2007): 123-128. https://doi.org/10.3892/ijmm.20.1.123