Diallyl disulfide accelerates adipogenesis in 3T3-L1 cells

  • Authors:
    • Ji-Hyun Lee
    • Kyung-Ah Kim
    • Kang-Beom Kwon
    • Eun-Kyung Kim
    • Young-Rae Lee
    • Mi-Young Song
    • Jeung-Hyun Koo
    • Sun-O Ka
    • Jin-Woo Park
    • Byung-Hyun Park
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/ijmm.20.1.59
  • Pages: 59-64
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Abstract

Adipocyte differentiation is regulated by the sequential activation of transcription factors such as the CAAT/enhancer binding protein and peroxisome proliferator- activated receptor-γ (PPAR-γ). Several recent studies have shown that regulators of chromatin structure are also involved in adipocyte differentiation. Here we investigated the effects of diallyl disulfide (DADS), an oil-soluble sulfur compound found in processed garlic and an inhibitor of histone deacetylase (HDAC), on adipogenesis. Treatment with DADS accelerated terminal differentiation of 3T3-L1 cells into adipocytes as evidenced by Oil red O staining and cellular triglyceride assay results. Notably, the inhibition of HDAC during the first 2 days was sufficient to stimulate adipogenesis. Western blot analysis revealed that DADS increased the level of acetylated histones H3 and H4. In addition, DADS increased the expression of adipogenesis-related genes; LPL, FAS, SREBP1c, aP2 and PPAR-γ, and decreased the expression of pref-1, a preadipocyte marker gene. Taken together, our results suggest that DADS affects adipocyte differentiation through histone acetylation at an early phase of adipocyte differentiation.

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July 2007
Volume 20 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Lee, J., Kim, K., Kwon, K., Kim, E., Lee, Y., Song, M. ... Park, B. (2007). Diallyl disulfide accelerates adipogenesis in 3T3-L1 cells. International Journal of Molecular Medicine, 20, 59-64. https://doi.org/10.3892/ijmm.20.1.59
MLA
Lee, J., Kim, K., Kwon, K., Kim, E., Lee, Y., Song, M., Koo, J., Ka, S., Park, J., Park, B."Diallyl disulfide accelerates adipogenesis in 3T3-L1 cells". International Journal of Molecular Medicine 20.1 (2007): 59-64.
Chicago
Lee, J., Kim, K., Kwon, K., Kim, E., Lee, Y., Song, M., Koo, J., Ka, S., Park, J., Park, B."Diallyl disulfide accelerates adipogenesis in 3T3-L1 cells". International Journal of Molecular Medicine 20, no. 1 (2007): 59-64. https://doi.org/10.3892/ijmm.20.1.59