Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells

  • Authors:
    • Heidrun Deissler
    • Eva-Maria Kuhn
    • Gabriele E. Lang
    • Helmut Deissler
  • View Affiliations

  • Published online on: November 1, 2007     https://doi.org/10.3892/ijmm.20.5.643
  • Pages: 643-652
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Abstract

Members of the tetraspanin protein family are modulators of several fundamental cellular processes in various cell types. However, expression and function of these proteins have not been studied in microvascular endothelial cells despite their (patho-)physiological importance. Western blotting, FACS or RT-PCR analyses confirmed that CD9 and other tetraspanins are expressed in immortalized microvascular endothelial cells of the bovine retina (iBREC). In subconfluent cultures, most of the detected CD9 was located intracellularly as well as in the plasma membrane at cell-cell contact sites and in long spike-like extensions, whereas cells in confluent cultures predominantly showed plasma membrane staining. In wound healing assays, CD9 delocalized from the plasma membrane to its intracellular compartment in cells located at the gap border, and the gap closure was retarded by the addition of an anti-CD9 antibody. Migration of iBREC towards fibronectin and their adhesion to fibronectin were also strongly inhibited in the presence of an anti-CD9 antibody whereas other anti-tetraspanin antibodies had no effect. In summary, iBREC express members of the tetraspanin family of which CD9 was demonstrated to have a function in migration and adhesion of these cells.

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november 2007
Volume 20 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Deissler H, Kuhn E, Lang GE and Deissler H: Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells. Int J Mol Med 20: 643-652, 2007.
APA
Deissler, H., Kuhn, E., Lang, G.E., & Deissler, H. (2007). Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells. International Journal of Molecular Medicine, 20, 643-652. https://doi.org/10.3892/ijmm.20.5.643
MLA
Deissler, H., Kuhn, E., Lang, G. E., Deissler, H."Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells". International Journal of Molecular Medicine 20.5 (2007): 643-652.
Chicago
Deissler, H., Kuhn, E., Lang, G. E., Deissler, H."Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells". International Journal of Molecular Medicine 20, no. 5 (2007): 643-652. https://doi.org/10.3892/ijmm.20.5.643