Evaluation of PRL-3 expression, and its correlation with angiogenesis and invasion in hepatocellular carcinoma

  • Authors:
    • Wen-Bo Zhao
    • Ying Li
    • Xin Liu
    • Ling-Yan Zhang
    • Xin Wang
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/ijmm_00000007
  • Pages: 187-192
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Abstract

Protein phosphatase of regenerating liver 3 (PRL-3) is a metastasis-associated phosphatase. Studies have shown that its overexpression increases cell motility and invasiveness. In this study, we aimed to investigate the expression of PRL-3 in hepatocellular carcinoma (HCC) tumor tissues and determine its correlations with matrix metalloproteinases (MMP-2, MMP-9) and E-cadherin in HCC. Paired cancerous and non-cancerous tissues were freshly collected from 42 primary HCC patients. PRL-3 expression at both mRNA and protein level was evaluated by real-time PCR, Western blot analysis and immunohistochemistry. The microvessel density (MVD) in HCC was detected with immunohistochemistry. The mRNA expression of MMP-2, MMP-9 and E-cadherin was analyzed by real-time PCR in search of correlations with PRL-3. We found that PRL-3 was significantly up-regulated in the HCC tumor tissues compared with corresponding noncancerous liver tissues (0.664±0.053 vs. 0.024±0.003, P<0.001). The mRNA level of PRL-3 in tissues was correlated with serum α-fetoprotein level, vascular invasion and metastasis (P<0.001). PRL-3 expression was closely related to MVD. Furthermore, we found a significant correlation between PRL-3 mRNA expression and MMP-2, MMP-9 and E-cadherin. Our results demonstrated that PRL-3 is up-regulated in HCC. It is strongly suggested that PRL-3 plays a key role in the angiogenesis and invasion of HCC. MMP-2, MMP-9 and E-cadherin might be involved in PRL-3 functions in HCC.

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August 2008
Volume 22 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhao W, Li Y, Liu X, Zhang L and Wang X: Evaluation of PRL-3 expression, and its correlation with angiogenesis and invasion in hepatocellular carcinoma. Int J Mol Med 22: 187-192, 2008.
APA
Zhao, W., Li, Y., Liu, X., Zhang, L., & Wang, X. (2008). Evaluation of PRL-3 expression, and its correlation with angiogenesis and invasion in hepatocellular carcinoma. International Journal of Molecular Medicine, 22, 187-192. https://doi.org/10.3892/ijmm_00000007
MLA
Zhao, W., Li, Y., Liu, X., Zhang, L., Wang, X."Evaluation of PRL-3 expression, and its correlation with angiogenesis and invasion in hepatocellular carcinoma". International Journal of Molecular Medicine 22.2 (2008): 187-192.
Chicago
Zhao, W., Li, Y., Liu, X., Zhang, L., Wang, X."Evaluation of PRL-3 expression, and its correlation with angiogenesis and invasion in hepatocellular carcinoma". International Journal of Molecular Medicine 22, no. 2 (2008): 187-192. https://doi.org/10.3892/ijmm_00000007