Melagenine modulates proliferation and differentiation of melanoblasts

  • Authors:
    • Dekuang Zhao
    • Yongwei Li
    • Ping Wang
    • Miaoni Zhou
    • Cuiping Guan
    • Xiaodong Wei
    • Yinsheng Wan
    • Aie Xu
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/ijmm_00000008
  • Pages: 193-197
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Abstract

Melagenine, extracted from human placenta, has been shown to be effective in treating patients with vitiligo, yet the mechanisms of melagenine in inducing the repigmentation of vitiligo patients have not been fully investigated. Recent studies have suggested that melagenine stimulates melanocyte proliferation and melanogenesis. In this study, we utilized the NCCmelb4M5 melanoblast cell line to investigate the effects of melagenine on proliferation and differentiation of immature melanocytes or melanoblasts. NCCmelb4M5 cells were treated with different concentrations of melagenine (50-400 μg/ml), and MTT assay was performed to evaluate the effects of melagenine on proliferation of melanoblasts. RT-PCR and Western blotting were used to determine the expression of c-KIT and tyrosinase (TYR). Our results show that melagenine stimulates proliferation of NCCmelb4M5 cells in a dose-dependent manner with an optimal concentration of 100 μg/ml. Multipolar and highly branched dendritic network, as well as cluster-like growing cell assembly were visible in melagenine-treated NCCmelb4M5 cells. Melagenine induced expression of c-KIT, TYR and MITF. Our results provide insights into the molecular mechanism of the beneficial effect of melagenine in the treatment of vitiligo.

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August 2008
Volume 22 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhao D, Li Y, Wang P, Zhou M, Guan C, Wei X, Wan Y and Xu A: Melagenine modulates proliferation and differentiation of melanoblasts. Int J Mol Med 22: 193-197, 2008
APA
Zhao, D., Li, Y., Wang, P., Zhou, M., Guan, C., Wei, X. ... Xu, A. (2008). Melagenine modulates proliferation and differentiation of melanoblasts. International Journal of Molecular Medicine, 22, 193-197. https://doi.org/10.3892/ijmm_00000008
MLA
Zhao, D., Li, Y., Wang, P., Zhou, M., Guan, C., Wei, X., Wan, Y., Xu, A."Melagenine modulates proliferation and differentiation of melanoblasts". International Journal of Molecular Medicine 22.2 (2008): 193-197.
Chicago
Zhao, D., Li, Y., Wang, P., Zhou, M., Guan, C., Wei, X., Wan, Y., Xu, A."Melagenine modulates proliferation and differentiation of melanoblasts". International Journal of Molecular Medicine 22, no. 2 (2008): 193-197. https://doi.org/10.3892/ijmm_00000008