EWS is a substrate of type I protein arginine methyltransferase, PRMT8

  • Authors:
    • Jun-Dal Kim
    • Koichiro Kako
    • Misako Kakiuchi
    • Gwi Gun Park
    • Akiyoshi Fukamizu
  • View Affiliations

  • Published online on: September 1, 2008     https://doi.org/10.3892/ijmm_00000024
  • Pages: 309-315
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Abstract

EWS, a pro-oncoprotein which is encoded by the Ewing sarcoma (EWS) gene, contains arginine-glycine-glycine repeats (RGG box) in its COOH-terminus. We previously found that the RGG box of EWS is a target for dimethylation catalyzed by protein arginine methyltransferases (PRMTs). Although it has been observed that arginine residues in EWS are dimethylated in vivo, the endogenous enzyme(s) responsible for this reaction have not been identified to date. In the present study, we determined that EWS was physically associated with PRMT8, the novel eighth member of the PRMT family, through the COOH-terminal region of EWS including RGG3 with the NH2-terminal region of PRMT8 encompassing the S-adenosyl-L-methionine binding domain, and that arginine residues in EWS were asymmetrically dimethylated by PRMT8 using amino acid analysis with thin-layer chromatography. These results suggested that EWS is a substrate for PRMT8, as efficient as for PRMT1.

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September 2008
Volume 22 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim J, Kako K, Kakiuchi M, Park GG and Fukamizu A: EWS is a substrate of type I protein arginine methyltransferase, PRMT8. Int J Mol Med 22: 309-315, 2008
APA
Kim, J., Kako, K., Kakiuchi, M., Park, G.G., & Fukamizu, A. (2008). EWS is a substrate of type I protein arginine methyltransferase, PRMT8. International Journal of Molecular Medicine, 22, 309-315. https://doi.org/10.3892/ijmm_00000024
MLA
Kim, J., Kako, K., Kakiuchi, M., Park, G. G., Fukamizu, A."EWS is a substrate of type I protein arginine methyltransferase, PRMT8". International Journal of Molecular Medicine 22.3 (2008): 309-315.
Chicago
Kim, J., Kako, K., Kakiuchi, M., Park, G. G., Fukamizu, A."EWS is a substrate of type I protein arginine methyltransferase, PRMT8". International Journal of Molecular Medicine 22, no. 3 (2008): 309-315. https://doi.org/10.3892/ijmm_00000024