Vitamin C inhibits p53-induced replicative senescence through suppression of ROS production and p38 MAPK activity

  • Authors:
    • Jee-Eun Kim
    • Dong-Hoon Jin
    • Soon-Duck Lee
    • Seung-Woo Hong
    • Jae-Sik Shin
    • Seung-Koo Lee
    • Da-Jung Jung
    • Jae-Seung Kang
    • Wang-Jae Lee
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/ijmm_00000068
  • Pages: 651-655
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Abstract

We previously reported that tumor cells expressing p53 increase intracellular levels of reactive oxygen species (ROS). In this study, we described an inhibitory effect of vitamin C on replicative senescence. Vitamin C was found to inhibit p53-induced senescence in human bladder cancer EJ cells. The senescence-like phenotype (SLP) induced by p53, which showed a morphological change and an irreversible cell cycle arrest, was not observed in vitamin C-treated EJ cells. In addition, vitamin C did not significantly affect normal cell proliferation. We investigated the molecular mechanisms of the inhibitory effect of vitamin C on the development of replicative senescence in EJ cells. We found that vitamin C inhibited this p53-induced ROS generation. Moreover, p38 kinase which was activated during p53-induced senescence was not observed in vitamin C-treated EJ cells. SB203580, a chemical inhibitor of p38 kinase, was found to consistently inhibit p53-induced senescence. Therefore, it is suggested that vitamin C inhibits p53-induced senescence by preventing ROS generation, which in turn leads to the activation of p38 MAPKinase. These results reveal the inhibitory mechanism of vitamin C on cellular senescence.

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November 2008
Volume 22 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim J, Jin D, Lee S, Hong S, Shin J, Lee S, Jung D, Kang J and Lee W: Vitamin C inhibits p53-induced replicative senescence through suppression of ROS production and p38 MAPK activity. Int J Mol Med 22: 651-655, 2008.
APA
Kim, J., Jin, D., Lee, S., Hong, S., Shin, J., Lee, S. ... Lee, W. (2008). Vitamin C inhibits p53-induced replicative senescence through suppression of ROS production and p38 MAPK activity. International Journal of Molecular Medicine, 22, 651-655. https://doi.org/10.3892/ijmm_00000068
MLA
Kim, J., Jin, D., Lee, S., Hong, S., Shin, J., Lee, S., Jung, D., Kang, J., Lee, W."Vitamin C inhibits p53-induced replicative senescence through suppression of ROS production and p38 MAPK activity". International Journal of Molecular Medicine 22.5 (2008): 651-655.
Chicago
Kim, J., Jin, D., Lee, S., Hong, S., Shin, J., Lee, S., Jung, D., Kang, J., Lee, W."Vitamin C inhibits p53-induced replicative senescence through suppression of ROS production and p38 MAPK activity". International Journal of Molecular Medicine 22, no. 5 (2008): 651-655. https://doi.org/10.3892/ijmm_00000068