Scaffold-dependent differentiation of human articular chondrocytes

  • Authors:
    • Werner Schlegel
    • Sylvia Nürnberger
    • Mathias Hombauer
    • Christian Albrecht
    • Vilmos Vécsei
    • Stefan Marlovits
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/ijmm_00000074
  • Pages:691-699
0

Abstract

Matrix-associated autologous chondrocyte transplantation (MACT) is a tissue-engineered approach for the treatment of cartilage defects and combines autologous chondrocytes seeded on biomaterials. The objective of the study is the analysis of growth and differentiation behaviour of human articular chondrocytes grown on three different matrices used for MACT. Human articular chondrocytes were kept in monolayer culture for 42 days and then seeded on matrices consisting of either collagen type I/III, hyaluronan, or gelatine. During the culture time of 4 weeks the constructs were analyzed weekly. Morphological criteria were studied by scanning and transmission electron microscopy. The expression of the main type collagens was analyzed by real-time PCR. The collagen type I/III matrix supported a differentiation that closely resembled the tissue organisation of native cartilage, but cell number and type II collagen synthesis were low and differentiation occurred rather late in the cultivation period. The hyaluronan matrix and the gelatine-based matrix supported a rather rapid differentiation, with a high number of cells and a relatively high amount of type II collagen, but there was no spatial assembly that mimicked native cartilage. These facts indicate that the nature of the matrix is of great influence in the differentiation behaviour of dedifferentiated chondrocytes.

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November 2008
Volume 22 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Schlegel, W., Nürnberger, S., Hombauer, M., Albrecht, C., Vécsei, V., & Marlovits, S. (2008). Scaffold-dependent differentiation of human articular chondrocytes. International Journal of Molecular Medicine, 22, 691-699. https://doi.org/10.3892/ijmm_00000074
MLA
Schlegel, W., Nürnberger, S., Hombauer, M., Albrecht, C., Vécsei, V., Marlovits, S."Scaffold-dependent differentiation of human articular chondrocytes". International Journal of Molecular Medicine 22.5 (2008): 691-699.
Chicago
Schlegel, W., Nürnberger, S., Hombauer, M., Albrecht, C., Vécsei, V., Marlovits, S."Scaffold-dependent differentiation of human articular chondrocytes". International Journal of Molecular Medicine 22, no. 5 (2008): 691-699. https://doi.org/10.3892/ijmm_00000074