Portable visible and near-infrared spectrophotometer for triglyceride measurements

  • Authors:
    • Takanori Kobayashi
    • Yukiko Hakariya Kato
    • Megumi Tsukamoto
    • Kazuyoshi Ikuta
    • Akikazu Sakudo
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  • Published online on: January 1, 2009     https://doi.org/10.3892/ijmm_00000103
  • Pages: 75-79
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Abstract

An affordable and portable machine is required for the practical use of visible and near-infrared (Vis-NIR) spectroscopy. A portable fruit tester comprising a Vis-NIR spectrophotometer was modified for use in the transmittance mode and employed to quantify triglyceride levels in serum in combination with a chemometric analysis. Transmittance spectra collected in the 600- to 1100-nm region were subjected to a partial least-squares regression analysis and leave-out cross-validation to develop a chemometrics model for predicting triglyceride concentrations in serum. The model yielded a coefficient of determination in cross-validation (R2VAL) of 0.7831 with a standard error of cross-validation (SECV) of 43.68 mg/dl. The detection limit of the model was 148.79 mg/dl. Furthermore, masked samples predicted by the model yielded a coefficient of determination in prediction (R2PRED) of 0.6856 with a standard error of prediction (SEP) and detection limit of 61.54 and 159.38 mg/dl, respectively. The portable Vis-NIR spectrophotometer may prove convenient for the measurement of triglyceride concentrations in serum, although before practical use there remain obstacles, which are discussed.

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January 2009
Volume 23 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kobayashi T, Kato YH, Tsukamoto M, Ikuta K and Sakudo A: Portable visible and near-infrared spectrophotometer for triglyceride measurements. Int J Mol Med 23: 75-79, 2009.
APA
Kobayashi, T., Kato, Y.H., Tsukamoto, M., Ikuta, K., & Sakudo, A. (2009). Portable visible and near-infrared spectrophotometer for triglyceride measurements. International Journal of Molecular Medicine, 23, 75-79. https://doi.org/10.3892/ijmm_00000103
MLA
Kobayashi, T., Kato, Y. H., Tsukamoto, M., Ikuta, K., Sakudo, A."Portable visible and near-infrared spectrophotometer for triglyceride measurements". International Journal of Molecular Medicine 23.1 (2009): 75-79.
Chicago
Kobayashi, T., Kato, Y. H., Tsukamoto, M., Ikuta, K., Sakudo, A."Portable visible and near-infrared spectrophotometer for triglyceride measurements". International Journal of Molecular Medicine 23, no. 1 (2009): 75-79. https://doi.org/10.3892/ijmm_00000103