Group B Streptococcus induces tyrosine phosphorylation of annexin V and glutathione S-transferase in human umbilical vein endothelial cells

  • Authors:
    • Gabriela S. Santos
    • Carla-Veronica Loureiro Y Penha
    • Ana Luiza Mattos-Guaraldi
    • Márcia Attias
    • Leila M. Lopes-Bezerra
    • Fernando C. Silva-Filho
    • Prescilla E. Nagao
  • View Affiliations

  • Published online on: September 1, 2009     https://doi.org/10.3892/ijmm_00000245
  • Pages: 393-399
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Abstract

Group B Streptococcus (GBS), a human pathogen that causes infection and invasive diseases in newborns, pregnant women and immunocompromised adults, has been shown to invade human umbilical vein endothelial cells (HUVECs). The objective of this study was to investigate the molecular mechanisms underlying GBS-HUVEC interaction, focusing specifically on the responsiveness of host protein tyrosine kinase (PTK). We found that GBS serotypes III and V induced actin reorganization and formation of stress fibers into HUVECs. Since rearrangements of the actin cytoskeleton into eukaryotic cells are usually associated with the activation of PTK, we decided to follow the expression of this class of kinases in the course of the interaction. Unexpectedly, treatment of HUVECs with genistein greatly increased both cytoadherence and intracellular viability, for all GBS strains studied. GBS increased tyrosine phosphorylation of two proteins with an apparent molecular mass of 35 and 23 kDa in HUVECs as demonstrated by Western blot analysis with anti-phosphotyrosine antibodies. Mass spectra analysis identified these proteins as annexin V and glutathione S-transferase. Studies are in progress to identify the role of these two proteins on GBS-HUVEC interaction.

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September 2009
Volume 24 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Santos GS, Loureiro Y Penha C, Mattos-Guaraldi A, Attias M, Lopes-Bezerra LM, Silva-Filho FC and Nagao PE: Group B Streptococcus induces tyrosine phosphorylation of annexin V and glutathione S-transferase in human umbilical vein endothelial cells. Int J Mol Med 24: 393-399, 2009
APA
Santos, G.S., Loureiro Y Penha, C., Mattos-Guaraldi, A., Attias, M., Lopes-Bezerra, L.M., Silva-Filho, F.C., & Nagao, P.E. (2009). Group B Streptococcus induces tyrosine phosphorylation of annexin V and glutathione S-transferase in human umbilical vein endothelial cells. International Journal of Molecular Medicine, 24, 393-399. https://doi.org/10.3892/ijmm_00000245
MLA
Santos, G. S., Loureiro Y Penha, C., Mattos-Guaraldi, A., Attias, M., Lopes-Bezerra, L. M., Silva-Filho, F. C., Nagao, P. E."Group B Streptococcus induces tyrosine phosphorylation of annexin V and glutathione S-transferase in human umbilical vein endothelial cells". International Journal of Molecular Medicine 24.3 (2009): 393-399.
Chicago
Santos, G. S., Loureiro Y Penha, C., Mattos-Guaraldi, A., Attias, M., Lopes-Bezerra, L. M., Silva-Filho, F. C., Nagao, P. E."Group B Streptococcus induces tyrosine phosphorylation of annexin V and glutathione S-transferase in human umbilical vein endothelial cells". International Journal of Molecular Medicine 24, no. 3 (2009): 393-399. https://doi.org/10.3892/ijmm_00000245