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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2009 Volume 24 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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Article

Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening

  • Authors:
    • M. Gnanasekar
    • F. G. Suleman
    • K. Ramaswamy
    • J. D. Caldwell

  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL 61107, USA. mgnanas@uic.edu
  • Pages: 421-426
    |
    Published online on: October 1, 2009
       https://doi.org/10.3892/ijmm_00000248
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Abstract

The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat proteins of the bacteriophage. T7 phage clones expressing normal human brain proteins (human normal brain phage-display cDNA expression library) were screened using SHBG as bait. The bound phage clones were then identified by DNA sequencing and by BLAST search analysis. Of the twenty binding proteins analyzed, three were found to be membrane-associated proteins: synaptosomal associated protein 25 (SNAP25), Thy-1 cell surface antigen and zonadhesin. Further studies will determine if the interactions of SHBG with these proteins have any role in the internalization and cell signaling events or whether they contribute to steroid delivery to specific cells.

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Copy and paste a formatted citation
Spandidos Publications style
Gnanasekar M, Suleman FG, Ramaswamy K and Caldwell JD: Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening. Int J Mol Med 24: 421-426, 2009.
APA
Gnanasekar, M., Suleman, F.G., Ramaswamy, K., & Caldwell, J.D. (2009). Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening. International Journal of Molecular Medicine, 24, 421-426. https://doi.org/10.3892/ijmm_00000248
MLA
Gnanasekar, M., Suleman, F. G., Ramaswamy, K., Caldwell, J. D."Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening". International Journal of Molecular Medicine 24.4 (2009): 421-426.
Chicago
Gnanasekar, M., Suleman, F. G., Ramaswamy, K., Caldwell, J. D."Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening". International Journal of Molecular Medicine 24, no. 4 (2009): 421-426. https://doi.org/10.3892/ijmm_00000248
Copy and paste a formatted citation
x
Spandidos Publications style
Gnanasekar M, Suleman FG, Ramaswamy K and Caldwell JD: Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening. Int J Mol Med 24: 421-426, 2009.
APA
Gnanasekar, M., Suleman, F.G., Ramaswamy, K., & Caldwell, J.D. (2009). Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening. International Journal of Molecular Medicine, 24, 421-426. https://doi.org/10.3892/ijmm_00000248
MLA
Gnanasekar, M., Suleman, F. G., Ramaswamy, K., Caldwell, J. D."Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening". International Journal of Molecular Medicine 24.4 (2009): 421-426.
Chicago
Gnanasekar, M., Suleman, F. G., Ramaswamy, K., Caldwell, J. D."Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening". International Journal of Molecular Medicine 24, no. 4 (2009): 421-426. https://doi.org/10.3892/ijmm_00000248
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