JNK and p38 inhibitors increase and decrease apoptosis, respectively, in pyrogallol-treated calf pulmonary arterial endothelial cells

  • Authors:
    • Yong Hwan Han
    • Hwa Jin Moon
    • Bo Ra You
    • Sung Zoo Kim
    • Suhn Hee Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: November 1, 2009     https://doi.org/10.3892/ijmm_00000284
  • Pages: 717-722
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Abstract

Pyrogallol (PG) as a polyphenol compound induces apoptosis in several types of cells. Here, we investigated the effects of MAPK inhibitors on PG-treated calf pulmonary artery endothelial cells (CPAEC) in relation to cell death, ROS and GSH. PG inhibited the growth of CPAEC and also induced cell death, which was accompanied by the loss of mitochondrial membrane potential (MMP; ΔΨm). PG decreased the ROS level and increased the GSH depleted cell number in CPAEC. JNK inhibitor intensified the growth inhibition by PG whereas p38 inhibitor attenuated the growth inhibition. While MEK and p38 inhibitors decreased CPAEC death by PG, JNK inhibitor increased. None of the MAPK inhibitors significantly increased ROS level in PG-treated CPAEC. JNK inhibitor increased GSH depleted cell number in PG-treated CPAEC whereas p38 inhibitor decreased the number. MAPK inhibitors differently affected cell growth, death, ROS and GSH levels in control CPACE. In conclusion, PG induced apoptosis via the loss of MMP (ΔΨm) in CPAEC, which is accompanied by GSH depletion. JNK and p38 inhibitors increased and decreased apoptosis in PG-treated CPAEC, respectively, which were correlated with GSH depletion.

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November 2009
Volume 24 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Han YH, Moon HJ, You BR, Kim SZ, Kim SH and Park WH: JNK and p38 inhibitors increase and decrease apoptosis, respectively, in pyrogallol-treated calf pulmonary arterial endothelial cells. Int J Mol Med 24: 717-722, 2009.
APA
Han, Y.H., Moon, H.J., You, B.R., Kim, S.Z., Kim, S.H., & Park, W.H. (2009). JNK and p38 inhibitors increase and decrease apoptosis, respectively, in pyrogallol-treated calf pulmonary arterial endothelial cells. International Journal of Molecular Medicine, 24, 717-722. https://doi.org/10.3892/ijmm_00000284
MLA
Han, Y. H., Moon, H. J., You, B. R., Kim, S. Z., Kim, S. H., Park, W. H."JNK and p38 inhibitors increase and decrease apoptosis, respectively, in pyrogallol-treated calf pulmonary arterial endothelial cells". International Journal of Molecular Medicine 24.5 (2009): 717-722.
Chicago
Han, Y. H., Moon, H. J., You, B. R., Kim, S. Z., Kim, S. H., Park, W. H."JNK and p38 inhibitors increase and decrease apoptosis, respectively, in pyrogallol-treated calf pulmonary arterial endothelial cells". International Journal of Molecular Medicine 24, no. 5 (2009): 717-722. https://doi.org/10.3892/ijmm_00000284