Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease

  • Authors:
    • Mizuki Ohira
    • Nobuhide Oshitani
    • Shuhei Hosomi
    • Kenji Watanabe
    • Hirokazu Yamagami
    • Kazunari Tominaga
    • Toshio Watanabe
    • Yasuhiro Fujiwara
    • Kiyoshi Maeda
    • Kosei Hirakawa
    • Tetsuo Arakawa
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/ijmm_00000300
  • Pages: 829-835
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Abstract

Crohn's disease is associated with increased permeability of the intestine even in quiescent patients. Increased intestinal permeability may cause dysregulated immunological responses in the intestinal mucosa that leads to chronic intestinal inflammation. Tight junction proteins contribute to intestinal permeability, and functional abnormality and dislocation of such proteins may cause increased intestinal permeability. We studied the expression of tight junction proteins Rab13, vasodilator-stimulated phosphoprotein (VASP), zonula occludin-1 (ZO-1), and F-actin in the intestinal epithelium of patients with inactive inflammatory bowel disease. Surgical samples were obtained from 10 controls (without inflammatory bowel disease), 10 patients with Crohn's disease and 7 patients with ulcerative colitis. F-actin was visualized with fluorescent phalloidin. Tight junction proteins were visualized by an immunofluorescence method. Rab13, VASP, and ZO-1 were found in apical tight junctions in normal epithelium but were dislocated to the basolateral position in patients with inactive Crohn's disease, whereas the structure of F-actin was maintained in inactive mucosa. In patients with ulcerative colitis, these tight junction proteins were not dislocated. Latent dislocation of tight junction proteins in the inactive mucosa of patients with Crohn's disease may permit the invasion of gut antigens to initiate and perpetuate altered immune response.

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December 2009
Volume 24 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ohira M, Oshitani N, Hosomi S, Watanabe K, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Hirakawa K, et al: Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease . Int J Mol Med 24: 829-835, 2009
APA
Ohira, M., Oshitani, N., Hosomi, S., Watanabe, K., Yamagami, H., Tominaga, K. ... Arakawa, T. (2009). Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease . International Journal of Molecular Medicine, 24, 829-835. https://doi.org/10.3892/ijmm_00000300
MLA
Ohira, M., Oshitani, N., Hosomi, S., Watanabe, K., Yamagami, H., Tominaga, K., Watanabe, T., Fujiwara, Y., Maeda, K., Hirakawa, K., Arakawa, T."Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease ". International Journal of Molecular Medicine 24.6 (2009): 829-835.
Chicago
Ohira, M., Oshitani, N., Hosomi, S., Watanabe, K., Yamagami, H., Tominaga, K., Watanabe, T., Fujiwara, Y., Maeda, K., Hirakawa, K., Arakawa, T."Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease ". International Journal of Molecular Medicine 24, no. 6 (2009): 829-835. https://doi.org/10.3892/ijmm_00000300