Fabrication of a microarray using a combination of the large circular sense and antisense DNA

  • Authors:
    • Kyung-Oh Doh
    • Yun-Han Lee
    • Kil-Hwan Han
    • Seok-Yong Uhm
    • Jong-Pil Kim
    • Yun-Ui Bae
    • Jeong-Hoh Park
    • Ik-Jae Moon
    • Jong-Gu Park
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijmm_00000320
  • Pages: 113-120
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Abstract

In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 cells. Subsequent functional analysis using an LC-antisense library of overexpressed genes identified 28 genes involved in A549 cell growth. These experiments demonstrated the proper features of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense and -antisense libraries for an effective functional validation of genes.

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January 2010
Volume 25 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y. ... Park, J. (2010). Fabrication of a microarray using a combination of the large circular sense and antisense DNA. International Journal of Molecular Medicine, 25, 113-120. https://doi.org/10.3892/ijmm_00000320
MLA
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y., Park, J., Moon, I., Park, J."Fabrication of a microarray using a combination of the large circular sense and antisense DNA". International Journal of Molecular Medicine 25.1 (2010): 113-120.
Chicago
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y., Park, J., Moon, I., Park, J."Fabrication of a microarray using a combination of the large circular sense and antisense DNA". International Journal of Molecular Medicine 25, no. 1 (2010): 113-120. https://doi.org/10.3892/ijmm_00000320